Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram Campus, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
Department of Oral Biology, Saveetha Dental College and Hospital, Chennai, Tamil Nadu, India.
Asian Pac J Cancer Prev. 2022 Aug 1;23(8):2797-2803. doi: 10.31557/APJCP.2022.23.8.2797.
To assess the expression of PGC1-α and PGC1-β in Oral squamous cell carcinoma in the presence and absence of hyperglycemia.
Fresh tissue samples were collected from 14 well differentiated OSCC patients with hyperglycemia, 14 OSCC patients without hyperglycemia and 14 healthy controls and subjected to quantitative real-time PCR to assess expression of PGC1-α and PGC1-β. The relative gene expression of PGC1-α and PGC1-β was calculated using the double delta Ct method. A two-fold difference was defined as over or under- expression. To further evaluate clinicopathological association, Independent t-test was employed.
The expression of both PGC1-α and PGC1-β were increased in OSCC patients when compared to healthy controls and similar findings were observed on calculating the fold change healthy controls and OSCC study groups. On assessing the expression of target genes within study groups, they did not present with significant fold change and the hyperglycemic status of the individual did not contribute to the expression of the target genes as P value obtained for PGC1-α and PGC1-β were >0.05.
The hyperglycemic status of the individual does not influence the expression of PGC1-α and PGC1-β in OSCC tissues and the cause for over-expression of the study targets in OSCC tissues must be further evaluated to assess their potential as possible candidates for targeted therapy in OSCC patients.
评估在存在和不存在高血糖的情况下，PGC1-α和 PGC1-β 在口腔鳞状细胞癌中的表达。
从 14 例高血糖分化良好的口腔鳞状细胞癌患者、14 例无高血糖的口腔鳞状细胞癌患者和 14 例健康对照者中采集新鲜组织样本，并进行定量实时 PCR 以评估 PGC1-α 和 PGC1-β 的表达。使用双 delta Ct 法计算 PGC1-α 和 PGC1-β 的相对基因表达。两倍的差异被定义为过度或低表达。为了进一步评估临床病理相关性，采用独立 t 检验。
与健康对照组相比，OSCC 患者的 PGC1-α 和 PGC1-β 表达均增加，在计算健康对照组和 OSCC 研究组的折叠变化时也观察到了类似的发现。在评估研究组内靶基因的表达时，它们没有表现出显著的折叠变化，个体的高血糖状态并没有导致靶基因的表达，因为获得的 PGC1-α 和 PGC1-β 的 P 值>0.05。
个体的高血糖状态不会影响 OSCC 组织中 PGC1-α 和 PGC1-β 的表达，必须进一步评估 OSCC 组织中研究靶标过度表达的原因，以评估它们作为 OSCC 患者靶向治疗可能候选物的潜力。
