The Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, DK-2100, Copenhagen, Denmark.
DTU Health Tech, Denmark's Technical University, Ørsteds Pl. 344, 108, 2800 Kgs., Lyngby, Denmark.
Sci Rep. 2022 Aug 29;12(1):14713. doi: 10.1038/s41598-022-18950-x.
Glioblastoma brain tumors form in the brain's white matter and remain one of the most lethal cancers despite intensive therapy and surgery. The complex morphology of these tumors includes infiltrative growth and gain of cell motility. Therefore, various brain-mimetic model systems have been developed to investigate invasion dynamics. Despite this, exactly how gradients of cell density, chemical signals and metabolites influence individual cells' migratory behavior remains elusive. Here we show that the gradient field induced by the spheroid-accelerates cells' invasion of the extracellular matrix. We show that cells are pushed away from the spheroid along a radial gradient, as predicted by a biased persistent random walk. Thus, our results grasp in a simple model the complex behavior of metastasizing cells. We anticipate that this well-defined and quantitative assay could be instrumental in the development of new anti-cancer strategies.
胶质母细胞瘤脑肿瘤形成于大脑的白质中，尽管进行了强化治疗和手术，但其仍然是最致命的癌症之一。这些肿瘤的复杂形态包括浸润性生长和细胞迁移能力的获得。因此，已经开发了各种类脑模型系统来研究入侵动力学。尽管如此，细胞密度、化学信号和代谢物梯度如何影响单个细胞的迁移行为仍然难以捉摸。在这里，我们表明，球体诱导的梯度场加速了细胞对细胞外基质的侵袭。我们表明，细胞沿着放射状梯度从球体被推开，正如偏向持续随机游走所预测的那样。因此，我们的结果在一个简单的模型中抓住了转移细胞的复杂行为。我们预计，这种定义明确和定量的测定方法可能有助于开发新的抗癌策略。
