Goll Johannes B, Jain Aarti, Jensen Travis L, Assis Rafael, Nakajima Rie, Jasinskas Algis, Coughlan Lynda, Cherikh Sami R, Gelber Casey E, Khan S, Huw Davies D, Meade Philip, Stadlbauer Daniel, Strohmeier Shirin, Krammer Florian, Chen Wilbur H, Felgner Philip L
The Emmes Company, LLC, Rockville, MD, USA.
Vaccine R&D Center, Department of Physiology and Biophysics, University of California-Irvine, Irvine, CA, USA.
NPJ Vaccines. 2022 Aug 30;7(1):103. doi: 10.1038/s41541-022-00524-7.
Current seasonal and pre-pandemic influenza vaccines induce short-lived predominantly strain-specific and limited heterosubtypic responses. To better understand how vaccine adjuvants AS03 and MF59 may provide improved antibody responses to vaccination, we interrogated serum from subjects who received 2 doses of inactivated monovalent influenza A/Indonesia/05/2005 vaccine with or without AS03 or MF59 using hemagglutinin (HA) microarrays (NCT01317758 and NCT01317745). The arrays were designed to reflect both full-length and globular head HA derived from 17 influenza A subtypes (H1 to H16 and H18) and influenza B strains. We observed significantly increased strain-specific and broad homo- and heterosubtypic antibody responses with both AS03 and MF59 adjuvanted vaccination with AS03 achieving a higher titer and breadth of IgG responses relative to MF59. The adjuvanted vaccine was also associated with the elicitation of stalk-directed antibody. We established good correlation of the array antibody responses to H5 antigens with standard HA inhibition and microneutralization titers.
当前的季节性流感疫苗和大流行前流感疫苗所诱导的抗体反应主要是针对特定毒株的，且持续时间较短，异源亚型反应有限。为了更好地了解疫苗佐剂AS03和MF59如何能改善疫苗接种后的抗体反应，我们使用血凝素（HA）微阵列对接受了2剂单价甲型流感病毒/印度尼西亚/05/2005灭活疫苗（有或没有AS03或MF59）的受试者的血清进行了检测（NCT01317758和NCT01317745）。这些微阵列旨在反映来自17种甲型流感病毒亚型（H1至H16和H18）以及乙型流感病毒株的全长和球状头部HA。我们观察到，使用AS03和MF59佐剂进行疫苗接种后，毒株特异性以及广泛的同源和异源亚型抗体反应均显著增加，与MF59相比，AS03佐剂疫苗接种产生的IgG反应滴度更高、广度更广。佐剂疫苗还与茎部定向抗体的诱导有关。我们确定了微阵列抗体对H5抗原的反应与标准HA抑制和微量中和滴度之间具有良好的相关性。
