外泌体微小RNA特征作为胆囊癌无创诊断的有效生物标志物。

Exosomal MicroRNA signature acts as an efficient biomarker for non-invasive diagnosis of gallbladder carcinoma.

作者信息

Yang Pinghua, Song Fengliang, Yang Xinwei, Yan Xingzhou, Huang Xinyu, Qiu Zhengjun, Wen Zhijian, Liang Chi, Xin Xianglei, Lei Zhengqin, Zhang Kecheng, Yang Jue, Liu Hu, Wang Hongcheng, Xiang Shijun, Li Liang, Zhang Baohua, Wang Hongyang

机构信息

Department of Biliary Tract Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, China.

School of Medicine, Nantong University, Nantong, Jiangsu Province, China.

出版信息

iScience. 2022 Jul 21;25(9):104816. doi: 10.1016/j.isci.2022.104816. eCollection 2022 Sep 16.

Through a three-step study that relies on biomarker discovery, training, and validation, we identified a set of five exosomal microRNAs (miRNAs) that can be used to evaluate the risk of gallbladder carcinoma (GBC), including miR-552-3p, miR-581, miR-4433a-3p, miR-496, and miR-203b-3p. When validated in 102 GBC patients and 112 chronic cholecystitis patients from multiple medical centers, the AUC of this combinatorial biomarker was 0.905, with a sensitivity of 81.37% and a specificity of 86.61%. The performance of this biomarker is superior to that of the standard biomarkers CA199 and CEA and is suited for GBC early diagnosis. The multi-clinicopathological features and prognosis of GBC patients were significantly associated with this biomarker. After building a miRNA-target gene regulation network, cell functions and signaling pathways regulated by these five miRNAs were examined. This biomarker signature can be used in the development of a noninvasive tool for GBC diagnosis, screening and prognosis prediction.

通过一项依赖生物标志物发现、训练和验证的三步研究，我们鉴定出一组可用于评估胆囊癌（GBC）风险的五个外泌体微小RNA（miRNA），包括miR-552-3p、miR-581、miR-4433a-3p、miR-496和miR-203b-3p。在来自多个医疗中心的102例GBC患者和112例慢性胆囊炎患者中进行验证时，该组合生物标志物的曲线下面积（AUC）为0.905，灵敏度为81.37%，特异性为86.61%。该生物标志物的性能优于标准生物标志物CA199和CEA，适用于GBC的早期诊断。GBC患者的多临床病理特征和预后与该生物标志物显著相关。构建miRNA-靶基因调控网络后，检测了这五个miRNA调控的细胞功能和信号通路。这种生物标志物特征可用于开发一种用于GBC诊断、筛查和预后预测的非侵入性工具。