Mussarat Sakina, Adnan Muhammad, Begum Shaheen, Ullah Riaz, Kowalczyk Alicja
Department of Botany, Kohat University of Science and Technology, Kohat 26000, Khyber Pakhtunkhwa, Pakistan.
Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan.
Evid Based Complement Alternat Med. 2022 Aug 22;2022:5977795. doi: 10.1155/2022/5977795. eCollection 2022.
A polyherbal formulation consisting of L., L. Kuntze, and (L.) Maton with a ratio of 10 : 5 : 2, respectively, was recommended for curing nausea, vomiting, and diarrhea. Experimental validation is crucial to affirm its therapeutic property leads toward the development of modified antidiarrheal agents. This research aimed to investigate the antidiarrheal efficacy of traditionally used polyherbal recipe in a castor oil-induced animal model. Moreover, the study also presents the elemental screening and toxicity of tested polyherbal recipe. Individual plant parts of the polyherbal recipe were mixed according to the traditional prescription ratio, and hydromethanolic extract was prepared by the cold maceration process. The antidiarrheal activity was assessed by castor oil induction method, charcoal meal test, and enteropooling procedure in Sprague-Dawley rats. Elemental analysis and subacute toxicity were carried out, followed by biochemical, hematological, and histopathological analyses. Polyherbal extract significantly delayed the diarrhea onset in a dose-dependent manner and showed marked inhibition at 200 and 400 mg/kg. Fecal weight was reduced significantly ( < 0.05) at 200 mg/kg (0.26 ± 0.25) in comparison with the control (1.63 ± 0.15). The diarrhea score was zero at a concentration of 200 and 400 mg/kg. Antienteropooling effect of the extract was greater than that of loperamide. Following subacute toxicity, all the treated rats were normal, survived, and showed no changes in behavior. There were no significant differences between values of blood parameters in both the control and extract-treated groups except a significant decrease in monocytes (control 8.4; polyherbal 2.2). Elemental analysis showed a slight increase in the amount of manganese (Mn, 8.076 ppm) as compared to the WHO recommended level (2 ppm). Traditionally used polyherbal recipe is effective and safe for combating diarrheal diseases. evidence supported the use, safety, and efficacy of the polyherbal recipe that has been used as an alternative medicine for diarrhea in the study area. Inhibition of castor oil-induced diarrhea and antisecretory effect of the studied polyherbal recipe makes it a potent antidiarrheal drug without no or limited toxic effects at the tested dose after further analysis.
一种由[植物名称1]、[植物名称2]和[植物名称3]按10∶5∶2的比例组成的多草药配方被推荐用于治疗恶心、呕吐和腹泻。实验验证对于确认其治疗特性从而推动改良止泻剂的开发至关重要。本研究旨在研究传统使用的多草药配方在蓖麻油诱导的动物模型中的止泻效果。此外，该研究还展示了受试多草药配方的元素筛查和毒性。多草药配方的各个植物部分按传统处方比例混合，通过冷浸法制备氢甲醇提取物。通过蓖麻油诱导法、炭末试验和肠积液程序在Sprague-Dawley大鼠中评估止泻活性。进行元素分析和亚急性毒性试验，随后进行生化、血液学和组织病理学分析。多草药提取物以剂量依赖性方式显著延迟腹泻发作，并在200和400mg/kg时表现出明显抑制作用。与对照组（1.63±0.15）相比，200mg/kg（0.26±0.25）时粪便重量显著降低（P<0.05）。在200和400mg/kg浓度下腹泻评分为零。提取物的抗肠积液作用大于洛哌丁胺。亚急性毒性试验后，所有受试大鼠均正常存活，行为无变化。除单核细胞显著减少（对照组8.4；多草药组2.2）外，对照组和提取物处理组的血液参数值之间无显著差异。元素分析表明，与世界卫生组织推荐水平（2ppm）相比，锰（Mn，8.076ppm）含量略有增加。传统使用的多草药配方在对抗腹泻疾病方面有效且安全。[具体证据]支持了该多草药配方在研究区域作为腹泻替代药物的使用、安全性和有效性。所研究的多草药配方对蓖麻油诱导的腹泻的抑制作用和抗分泌作用使其成为一种有效的止泻药物，经进一步分析，在受试剂量下无或仅有有限的毒性作用。
