Devanathadesikan Seshadri Vidya, Vijayaraghavan P, Kim Y-O, Kim H-J, Ahmed Al-Ghamdi Abdullah, Elshikh Mohamed S, Al-Dosary Monerah A, Alsubaie Qasi D
Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam bin Abdul Aziz University, Al-Kharj, Saudi Arabia.
Bioprocessing Engineering Division, Smykon Biotech Pvt. LtD, Nagercoil, Kanyakumari District, Tamil Nadu, India.
Saudi J Biol Sci. 2020 Jun;27(6):1475-1481. doi: 10.1016/j.sjbs.2020.04.005. Epub 2020 Apr 10.
Various metabolites exist in the medicinal plants have lot of potential to cure various diseases and disorders. Plants such as, , , and were collected from Western Ghats, Tamilnadu, India. Phytochemicals were extracted from these plants using various organic solvents and tested against Gram-positive and Gram-negative bacteria. The phytochemicals such as, carbohydrate, alkaloids, steroids, saponins, flavonoids and tannin were detected from these medicinal plants. Among the extracts, methanol showed potent activity and this solvent was used to extract polyherbal medicinal plants. Methanol extract of was found to be highly active against (27 ± 2 mm), (19 ± 3 mm) and (18 ± 2 mm). Ethyl acetate extract showed high activity against (24 ± 2 mm), (22 ± 3 mm) and (20 ± 1 mm). These three plants were taken at 1:1:1 ratio and extracted with methanol at 1:10 ratio and synergistic activity was tested against bacterial pathogens. Synergistic activity of polyherbal extract was analyzed. The extracted crude herbal medicine was found to be effective against sp. and . The zone of inhibition was 33 ± 3 mm, 17 ± 2 mm, 22 ± 2 mm, 40 ± 2 mm, 33 ± 1 mm and 38 ± 2 mm zone of inhibition against , , , , and sp. Polyherbal extract was found to be highly effective against and sp. MIC values of polyherbal extract ranged from 29 ± 2.5 µg/ml to 34 ± 2.5 µg/ml. MIC value was found to be less against and was high against . Antioxidant property varied between 49 ± 3% and 95.3 ± 2%. At 20 µg/ml antioxidant activity was reported as 49 ± 3% and it was increased at higher concentrations of polyherbal extract. Two cell lines (HeLa and MCF cell lines) were selected to analyze cytotoxic activity of polyherbal extract. The methanol extract of polyherbal fraction showed cytotoxicity against these two cell lines. The LC50 value was 467 ± 2.9 µg/ml against HeLa cell line and >800 µg/ml against MCF-7 cell lines. The polyherbal extract showed antibacterial, antioxidant and anticancer activities.
药用植物中存在的各种代谢物具有治疗各种疾病和病症的巨大潜力。从印度泰米尔纳德邦西高止山脉采集了诸如[此处原文缺失植物名称]等植物。使用各种有机溶剂从这些植物中提取植物化学物质，并针对革兰氏阳性和革兰氏阴性细菌进行测试。从这些药用植物中检测到了碳水化合物、生物碱、类固醇、皂苷、黄酮类化合物和单宁等植物化学物质。在提取物中，甲醇显示出强效活性，该溶剂被用于提取多草药植物。[此处原文缺失植物名称]的甲醇提取物对[此处原文缺失细菌名称]（27±2毫米）、[此处原文缺失细菌名称]（19±3毫米）和[此处原文缺失细菌名称]（18±2毫米）具有高度活性。乙酸乙酯提取物对[此处原文缺失细菌名称]（24±2毫米）、[此处原文缺失细菌名称]（22±3毫米）和[此处原文缺失细菌名称]（20±1毫米）显示出高活性。将这三种植物按1:1:1的比例混合，以1:10的比例用甲醇提取，并测试其对细菌病原体的协同活性。分析了多草药提取物的协同活性。发现提取的粗草药对[此处原文缺失细菌名称]和[此处原文缺失细菌名称]有效。抑菌圈直径分别为33±3毫米、17±2毫米、22±2毫米、40±2毫米、33±1毫米和38±2毫米，分别针对[此处原文缺失细菌名称]、[此处原文缺失细菌名称]、[此处原文缺失细菌名称]、[此处原文缺失细菌名称]、[此处原文缺失细菌名称]和[此处原文缺失细菌名称]。发现多草药提取物对[此处原文缺失细菌名称]和[此处原文缺失细菌名称]非常有效。多草药提取物的最低抑菌浓度（MIC）值范围为29±2.5微克/毫升至34±2.5微克/毫升。发现对[此处原文缺失细菌名称]的MIC值较低，对[此处原文缺失细菌名称]的MIC值较高。抗氧化性能在49±3%至95.3±2%之间变化。在20微克/毫升时，抗氧化活性报告为49±3%，在多草药提取物浓度较高时活性增加。选择两种细胞系（HeLa和MCF细胞系）来分析多草药提取物的细胞毒性活性。多草药部分的甲醇提取物对这两种细胞系显示出细胞毒性。对HeLa细胞系的半数致死浓度（LC50）值为467±2.9微克/毫升，对MCF - 7细胞系大于800微克/毫升。多草药提取物显示出抗菌、抗氧化和抗癌活性。
