来自基多、瓜亚基尔和科塔卡奇地区的三个厄瓜多尔蜂胶样品的化学特征及抗利什曼原虫活性
Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions.
作者信息
Cuesta-Rubio Osmany, Campo Fernández Mercedes, Márquez Hernández Ingrid, Jaramillo Carmita Gladys Jaramillo, González Victor Hugo, Montes De Oca Porto Rodny, Marrero Delange David, Monzote Fidalgo Lianet, Piccinelli Anna Lisa, Campone Luca, Rastrelli Luca
机构信息
Universidad Técnica de Machala. Km. 5 1/2 Panamericana Vía a Pasaje, Machala, Ecuador.
Instituto de Medicina del Deporte, Laboratorio Antidoping, Calle 100 y Aldabó, CP 12 10800 La Habana, Cuba.
出版信息
Fitoterapia. 2017 Jul;120:177-183. doi: 10.1016/j.fitote.2017.06.016. Epub 2017 Jun 19.
Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC-MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC=17.1±1.7μg/mL). In the literature this is the first study on propolis from Ecuador.
从厄瓜多尔的不同地区（基多、瓜亚基尔和科塔卡奇）采集了三个蜂胶样品，并制备了它们的甲醇提取物。薄层色谱（TLC）和核磁共振（NMR）数据提供的初步信息使我们能够确定两种主要类型的蜂胶：富含黄酮类化合物的科塔卡奇蜂胶样品（CPS），以及以三萜醇和乙酰三萜为主要成分的基多和瓜亚基尔样品（QPS和GPS）。基于反相高效液相色谱（RP-HPLC）制备程序和核磁共振结构测定（CPS）以及气相色谱-质谱联用（GC-MS）分析（QPS和GPS）的两种不同方法成功用于它们主要化合物的化学表征。所有三种蜂胶提取物都能够抑制亚马逊利什曼原虫的生长，但富含黄酮类化合物的蜂胶样品活性最高（IC=17.1±1.7μg/mL）。在文献中，这是对厄瓜多尔蜂胶的首次研究。
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