Division of Immunology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Division of Immunology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Clin Immunol. 2022 Oct;243:109106. doi: 10.1016/j.clim.2022.109106. Epub 2022 Aug 30.
Multisystem inflammatory syndrome in children (MIS-C) is a severe complication of SARS-CoV-2 infections that occurs in the pediatric population. We sought to characterize T cell responses in MIS-C compared to COVID-19 and pediatric hyperinflammatory syndromes. MIS-C was distinct from COVID-19 and hyperinflammatory syndromes due to an expansion of T cells expressing TRBV11-2 that was not associated with HLA genotype. Children diagnosed with MIS-C, but who were negative for SARS-CoV-2 by PCR and serology, did not display Vβ skewing. There was no difference in the proportion of T cells that became activated after stimulation with SARS-CoV-2 peptides in children with MIS-C compared to convalescent COVID-19. The frequency of SARS-CoV-2-specific TCRs and the antigens recognized by these TCRs were comparable in MIS-C and COVID-19. Expansion of Vβ11-2 T cells was a specific biomarker of MIS-C patients with laboratory confirmed SARS-CoV-2 infections. Children with MIS-C had robust antigen-specific T cell responses to SARS-CoV-2.
儿童多系统炎症综合征（MIS-C）是儿童 SARS-CoV-2 感染的严重并发症。我们试图比较 MIS-C 与 COVID-19 和儿童炎症性综合征之间的 T 细胞反应。MIS-C 与 COVID-19 和炎症性综合征不同，因为表达 TRBV11-2 的 T 细胞扩增与 HLA 基因型无关。通过 PCR 和血清学检测被诊断为 MIS-C 但 SARS-CoV-2 阴性的儿童没有显示 Vβ 偏倚。与恢复期 COVID-19 相比，MIS-C 儿童在 SARS-CoV-2 肽刺激后被激活的 T 细胞比例没有差异。MIS-C 和 COVID-19 中，SARS-CoV-2 特异性 TCR 的频率和这些 TCR 识别的抗原是可比的。Vβ11-2 T 细胞的扩增是实验室确诊 SARS-CoV-2 感染的 MIS-C 患者的特异性生物标志物。MIS-C 患儿对 SARS-CoV-2 具有强烈的抗原特异性 T 细胞反应。
