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一种传统中草药的完整叶绿体基因组序列

The complete chloroplast genome sequence of a traditional Chinese medicinal herb.

作者信息

Wang Xinhong, Li Lulu, Ma Xiaojun, Su He, Shu Jing

机构信息

College of Forestry Engineering, Shandong Agriculture and Engineering University, Jinan, China.

The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.

出版信息

Mitochondrial DNA B Resour. 2022 Aug 28;7(8):1559-1561. doi: 10.1080/23802359.2022.2098855. eCollection 2022.

Abstract

C. B. Clarke is traditionally used for the treatment of inflammation, typhoid fever, injuries due to falls, abdominal pain, malaria, and acute appendicitis ulcers in China and Korea. In this study, the complete chloroplast genome of was sequenced using the Illumina HiSeq X-Ten platform. It had a circular shape and a length of 158,940 bp, with 38.51% GC content. It contained a large single-copy region of 87,641 bp, a small single-copy region of 12,807 bp, and two inverted repeat regions of 29,246 bp. In total, 128 different genes, including 83 protein-coding genes, 37 distinct tRNA genes, and eight rRNA genes, were identified. Maximum-likelihood phylogenomic analysis showed that is closely related to and in the Valerianaceae family. There were 4535 variable sites, 157,354 conserved sites, and 3085 singleton sites in the six chloroplast genomes.

摘要

在中国和韩国,传统上使用C. B. Clarke来治疗炎症、伤寒、跌倒所致损伤、腹痛、疟疾和急性阑尾炎溃疡。在本研究中,使用Illumina HiSeq X-Ten平台对其完整叶绿体基因组进行了测序。它呈环状,长度为158,940 bp,GC含量为38.51%。它包含一个87,641 bp的大单拷贝区域、一个12,807 bp的小单拷贝区域和两个29,246 bp的反向重复区域。总共鉴定出128个不同的基因,包括83个蛋白质编码基因、37个不同的tRNA基因和8个rRNA基因。最大似然系统发育基因组分析表明,它与缬草科中的 和 密切相关。在六个叶绿体基因组中,有4535个可变位点、157,354个保守位点和3085个单例位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac2d/9427040/8213c08fcf7e/TMDN_A_2098855_F0001_B.jpg

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