Yang Wenling, Wang Baitao, Lei Gao, Chen Guocan, Liu Dehai
Key Laboratory of Microbial Engineering at the Institute of Biology, Institute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, China.
Front Bioeng Biotechnol. 2022 Aug 16;10:974646. doi: 10.3389/fbioe.2022.974646. eCollection 2022.
RNAi technology, known as a revolutionary technology in the history of pesticides, has been identified as a very promising novel approach for crop protection, which is of great significance for achieving the sustainable agricultural development of the United Nations Food and Agriculture Organization. Although many studies have shown that RNA biopesticides have strong application prospects, its stability seriously restricts the commercial use. As the core component of RNAi, double-stranded RNA (dsRNA) is unstable in its natural form. Therefore, how to ensure the stability of dsRNA is one of the most significant challenges in realizing the commercial use of RNA biopesticides. Nanomaterials such as cationic polymers and lipofectamine can improve the stability of dsRNA in the environment, which has been proved. This paper reviews the recent research progress of nanomaterials that can be used to improve the environmental stability of dsRNA, and discusses the advantages and limitations of different nanomaterials combined with dsRNA, which provides reference for the selection of dsRNA nanoformulations.
RNA干扰技术被誉为农药史上的一项革命性技术，已被认定为一种极具前景的新型作物保护方法，这对实现联合国粮食及农业组织的可持续农业发展具有重要意义。尽管许多研究表明RNA生物农药具有强大的应用前景，但其稳定性严重限制了其商业应用。作为RNA干扰的核心成分，双链RNA（dsRNA）以其天然形式存在时不稳定。因此，如何确保dsRNA的稳定性是实现RNA生物农药商业应用面临的最重大挑战之一。阳离子聚合物和脂质体等纳米材料能够提高dsRNA在环境中的稳定性，这一点已得到证实。本文综述了可用于提高dsRNA环境稳定性的纳米材料的最新研究进展，并探讨了不同纳米材料与dsRNA结合的优缺点，为dsRNA纳米制剂的选择提供参考。
