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基于转导嗅鞘细胞的新型基因-细胞构建物治疗脊髓损伤。

Obtaining a New Gene-Cell Construct Based on Transduced Olfactory Ensheathing Cells for the Treatment of Spinal Cord Injuries.

机构信息

V. P. Serbsky National Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.

National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2022 Aug;173(4):529-533. doi: 10.1007/s10517-022-05576-2. Epub 2022 Sep 5.

DOI:10.1007/s10517-022-05576-2
PMID:36058977
Abstract

We developed a viral vector Ad5/35-CAG-mBDNF expressing the mature form of BDNF (mBDNF). On the basis of olfactory ensheathing cells transduced with this adenovector, a new gene-cell construct was obtained. In experiments in vitro, high viability of the transduced olfactory ensheathing cells and enhanced secretion of BDNF by these cells were observed. It is possible that a new gene-cell construct will significantly increase the regenerative effects of transplanted olfactory ensheathing cells.

摘要

我们构建了一种表达成熟脑源性神经营养因子（mBDNF）的腺病毒载体 Ad5/35-CAG-mBDNF。在此腺病毒载体转导的嗅鞘细胞的基础上，获得了一种新型基因细胞构建体。在体外实验中，观察到转导的嗅鞘细胞具有高存活率和增强的 BDNF 分泌。新型基因细胞构建体有可能显著增强移植的嗅鞘细胞的再生效果。

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本文引用的文献

1
Study of the Therapeutic Efficiency of Transduced Olfactory Ensheathing Cells in Spinal Cord Cysts.转导嗅鞘细胞治疗脊髓囊肿的疗效研究
Stem Cells Dev. 2022 Jan;31(1-2):9-17. doi: 10.1089/scd.2021.0265. Epub 2021 Dec 28.
2
A new insight into aggregation of oncolytic adenovirus Ad5-delta-24-RGD during CsCl gradient ultracentrifugation.在 CsCl 梯度超速离心过程中对溶瘤腺病毒 Ad5-delta-24-RGD 聚集的新认识。
Sci Rep. 2021 Aug 9;11(1):16088. doi: 10.1038/s41598-021-94573-y.
3
The Effect of Transplantation of Olfactory Ensheathing Cells on the Size of Posttraumatic Spinal Cord Cysts.
嗅鞘细胞移植对创伤后脊髓囊肿大小的影响。
Bull Exp Biol Med. 2021 May;171(1):122-126. doi: 10.1007/s10517-021-05183-7. Epub 2021 May 28.
4
Viral vector platforms within the gene therapy landscape.病毒载体平台在基因治疗领域中的应用。
Signal Transduct Target Ther. 2021 Feb 8;6(1):53. doi: 10.1038/s41392-021-00487-6.
5
Immune Responses to Viral Gene Therapy Vectors.病毒基因治疗载体的免疫反应。
Mol Ther. 2020 Mar 4;28(3):709-722. doi: 10.1016/j.ymthe.2020.01.001. Epub 2020 Jan 10.
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Moving beyond the glial scar for spinal cord repair.超越脊髓修复的神经胶质瘢痕。
Nat Commun. 2019 Aug 28;10(1):3879. doi: 10.1038/s41467-019-11707-7.
7
Beyond Trophic Factors: Exploiting the Intrinsic Regenerative Properties of Adult Neurons.超越营养因子：利用成年神经元的内在再生特性
Front Cell Neurosci. 2019 Apr 5;13:128. doi: 10.3389/fncel.2019.00128. eCollection 2019.
8
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PLoS One. 2017 Dec 11;12(12):e0188967. doi: 10.1371/journal.pone.0188967. eCollection 2017.
9
Olfactory ensheathing cells but not fibroblasts reduce the duration of autonomic dysreflexia in spinal cord injured rats.嗅鞘细胞而非成纤维细胞可缩短脊髓损伤大鼠自主神经反射障碍的持续时间。
Auton Neurosci. 2016 Dec;201:17-23. doi: 10.1016/j.autneu.2016.08.015. Epub 2016 Aug 24.
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