V. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
National Medical Research Centre of Cardiology named after academician E. I. Chazov, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2024 Aug;177(4):552-558. doi: 10.1007/s10517-024-06222-9. Epub 2024 Sep 12.
A new gene-cell construct expressing nerve growth factor (NGF) has been developed. After obtaining engineered adenovectors Ad5-RGD-CAG-NGF and Ad5-RGD-CAG-EGFP, transduction efficiency and transgene expression were studied and multiplicity of infection was determined. The efficacy of transduced human olfactory ensheathing cells expressing NGF in restoring motor activity in rats has been shown in a limited period of time. Improved rat hindlimb mobility and cyst size reduction after gene-cell construct transplantation were more likely due to the cellular component of the construct.
一种表达神经生长因子（NGF）的新型基因-细胞构建体已经开发出来。在获得工程腺病毒载体 Ad5-RGD-CAG-NGF 和 Ad5-RGD-CAG-EGFP 后，研究了转导效率和转基因表达，并确定了感染复数。在有限的时间内，表达 NGF 的转导人嗅鞘细胞在恢复大鼠运动活动中的功效已经得到了证明。基因-细胞构建体移植后大鼠后肢活动能力的提高和囊肿体积的减小更可能是由于构建体的细胞成分。
