V. P. Serbsky National Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
M. V. Lomonosov Moscow State University, Moscow, Russia.
Bull Exp Biol Med. 2022 Mar;172(5):617-621. doi: 10.1007/s10517-022-05446-x. Epub 2022 Mar 30.
A gene-cell construct based on rat olfactory mucosa ensheathing cells transduced with an adenoviral vector encoding a mature form of brain neurotrophic factor (mBDNF) was transplanted into post-traumatic cysts of rat spinal cord. Transplantation of the gene-cell construct improved motor activity of the hind limbs and reduced the size of cysts in some animals. However, comparison of the effects of transduced and non-transduced ensheathing cells revealed no significant differences. In parallel in vitro experiments, a decrease in the proliferation of transduced cells compared to non-transduced cells was observed. It is likely that mBDNF reduces proliferation of transduced cells, which can affect their efficiency. The therapeutic efficacy of the new gene-cell construct is most likely provided by the cellular component.
基于转导腺病毒载体编码成熟脑源性神经营养因子（mBDNF）的大鼠嗅黏膜神经鞘细胞的基因-细胞构建体被移植到大鼠脊髓创伤后囊肿中。移植基因-细胞构建体改善了后肢的运动活动，并减少了一些动物囊肿的大小。然而，转导和未转导神经鞘细胞的效果比较没有显示出显著差异。在平行的体外实验中，与未转导细胞相比，转导细胞的增殖减少。mBDNF 可能降低了转导细胞的增殖，这可能会影响它们的效率。新型基因-细胞构建体的治疗效果很可能是由细胞成分提供的。
