Ayvazyan Arpine, Deutsch Lenard, Zidorn Christian, Kircher Brigitte, Çiçek Serhat S
Department of Pharmaceutical Biology, Kiel University, Kiel, Germany.
Tyrolean Cancer Research Institute, Innsbruck, Austria.
Front Plant Sci. 2023 Dec 4;14:1269710. doi: 10.3389/fpls.2023.1269710. eCollection 2023.
The roots of are the source of the traditional Chinese medicine danshen and the class of tanshinones, particular quinoid -diterpenoids of the abietane type. Of these compounds, cryptotanshinone, dihydrotanshinone I, tanshinone I, and tanshinone IIA, have been extensively studied for their anticancer potential, not only but as well because of their high abundance in and their thus easy availability. However, also additional species are known to contain tanshinones, mainly such of the subgenus , of which is the only species widely occurring in Europe. Using UHPLC-DAD-MS, the tanshinone profile of roots collected from two different locations was compared to the profile in roots. In addition, tanshinone IIA and another six diterpenoids from were investigated for their antiproliferative and cytotoxic potential against MDA-MB-231 and HL-60 cells. Apart from dihydrotanshinone I, which has been previously characterized due to its anticancer properties, we determined danshenol A as a highly antiproliferative and cytotoxic agent, significantly surpassing the effects of dihydrotanshinone I. With regard to the diterpenoid profile, showed a higher concentration for most of the tanshinones, except for (+)-danshexinkun A, which was present in comparable amounts in both species. Danshenol A, in contrast, was only present in as were dehydroabietic acid and (+)-pisiferic acid. The results of our study underlines the long traditional use of danshen due to its high amount on tanshinones, but also demonstrates the potential value of investigating closely related species for the discovery of new biologically active lead compounds.
[植物名称]的根是传统中药丹参以及丹参酮类化合物的来源，特别是松香烷型的醌类二萜化合物。在这些化合物中，隐丹参酮、二氢丹参酮I、丹参酮I和丹参酮IIA因其抗癌潜力而受到广泛研究，这不仅是因为它们在[植物名称]中含量高且易于获取。然而，已知还有其他[植物名称]物种也含有丹参酮，主要是[亚属名称]的物种，其中[植物名称]是唯一在欧洲广泛分布的物种。使用超高效液相色谱-二极管阵列检测器-质谱联用仪（UHPLC-DAD-MS），比较了从两个不同地点采集的[植物名称]根的丹参酮谱与[另一植物名称]根中的谱。此外，研究了丹参酮IIA和来自[植物名称]的另外六种二萜化合物对MDA-MB-231和HL-60细胞的抗增殖和细胞毒性潜力。除了先前因其抗癌特性而被表征的二氢丹参酮I外，我们确定丹参醇A是一种高度抗增殖和细胞毒性的药物，其效果显著超过二氢丹参酮I。关于二萜谱，[植物名称]中大多数丹参酮的浓度较高，但（+）-丹参新醌A除外，它在两种物种中的含量相当。相比之下，丹参醇A仅存在于[植物名称]中，脱氢枞酸和（+）-海松酸也是如此。我们的研究结果强调了丹参因其高含量的丹参酮而长期以来的传统用途，但也证明了研究密切相关物种以发现新的生物活性先导化合物的潜在价值。
