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从牡蛎(太平洋牡蛎)中复苏霍乱弧菌的升温法

Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas).

作者信息

DePaola A, Kaysner C A, McPhearson R M

出版信息

Appl Environ Microbiol. 1987 May;53(5):1181-2. doi: 10.1128/aem.53.5.1181-1182.1987.

DOI:10.1128/aem.53.5.1181-1182.1987
PMID:3606095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC203830/
Abstract

Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rates of isolation (P less than 0.05) and recovery (P less than 0.01) of V. cholerae from oysters were observed at 42 degrees C.

摘要

在从不同临床和环境来源分离出的222株霍乱弧菌中,219株在42摄氏度下过夜培养时能在碱性蛋白胨肉汤中产生可见生长。在比较42摄氏度和35摄氏度培养温度下增菌效果的现场试验中,在42摄氏度时从牡蛎中分离出霍乱弧菌的比率(P<0.05)和回收率(P<0.01)显著更高。

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本文引用的文献

1
[A NEW SELECTIVE ISOLATION MEDIUM FOR THE VIBRIO GROUP; ON A MODIFIED NAKANISHI'S MEDIUM (TCBS AGAR MEDIUM)].[用于弧菌属的一种新型选择性分离培养基;关于改良的中西氏培养基(TCBS琼脂培养基)]
Nihon Saikingaku Zasshi. 1963 Nov;18:387-92. doi: 10.3412/jsb.18.387.
2
Cholera--a possible endemic focus in the United States.霍乱——美国境内一个可能的地方性疫源地。
N Engl J Med. 1980 Feb 7;302(6):305-9. doi: 10.1056/NEJM198002073020601.
3
Characterization of Vibrio cholerae isolated from oysters.从牡蛎中分离出的霍乱弧菌的特性研究。
Appl Environ Microbiol. 1981 Jun;41(6):1475-8. doi: 10.1128/aem.41.6.1475-1478.1981.
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Isolation of Vibrio cholerae serotype O1 from the eastern oyster, Crassostrea virginica.从美国东海岸牡蛎(弗吉尼亚牡蛎)中分离出霍乱弧菌O1血清型。
Appl Environ Microbiol. 1981 Feb;41(2):559-60. doi: 10.1128/aem.41.2.559-560.1981.
5
Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries.马里兰州和路易斯安那州河口霍乱弧菌O1血清型的出现情况。
Appl Environ Microbiol. 1981 Feb;41(2):555-8. doi: 10.1128/aem.41.2.555-558.1981.
6
Non-O group 1 Vibrio cholerae gastroenteritis in the United States: clinical, epidemiologic, and laboratory characteristics of sporadic cases.美国非O1群霍乱弧菌性肠胃炎:散发病例的临床、流行病学及实验室特征
Ann Intern Med. 1981 May;94(5):656-8. doi: 10.7326/0003-4819-94-5-656.
7
Seasonal variations of Vibrio cholerae (non-O1) isolated from California coastal waters.从加利福尼亚沿海水域分离出的霍乱弧菌(非O1群)的季节性变化。
Appl Environ Microbiol. 1984 Jun;47(6):1243-5. doi: 10.1128/aem.47.6.1243-1245.1984.
8
Laboratory methods in cholera: isolation of Vibrio cholerae (el tor and classical) on TCBS medium in minimally equipped laboratories.霍乱的实验室方法:在设备简陋的实验室中,于硫代硫酸盐-柠檬酸盐-胆盐-蔗糖(TCBS)培养基上分离霍乱弧菌(埃尔托生物型和古典生物型)
Trans R Soc Trop Med Hyg. 1968;62(5):693-9. doi: 10.1016/0035-9203(68)90121-1.
9
A case of cholera in Texas, 1973.1973年,得克萨斯州的一例霍乱病例。
Am J Epidemiol. 1974 Dec;100(6):487-98. doi: 10.1093/oxfordjournals.aje.a112061.
10
Ecology, serology, and enterotoxin production of Vibrio cholerae in Chesapeake Bay.切萨皮克湾霍乱弧菌的生态学、血清学及肠毒素产生情况
Appl Environ Microbiol. 1979 Jan;37(1):91-103. doi: 10.1128/aem.37.1.91-103.1979.