Green Breann V, Ford Travis W, Goldsborrough Heather, Abdelmotalab Mohamed, Ristvey Andrew G, Sauder Deborah G, Volkis Victoria V
Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, Maryland 21853, United States.
University of Maryland Extension, Wye Research & Education Center, P.O. Box 169, Queenstown, Maryland 21658-0169, United States.
ACS Omega. 2022 Aug 18;7(34):29877-29885. doi: 10.1021/acsomega.2c02785. eCollection 2022 Aug 30.
Antioxidants play a vital role in the human body by defending cells from damage caused by free radicals, highly reactive products of oxidation reactions. A major source of antioxidants is fruits and vegetables. , a breed created at the end of the 19th century by crossbreeding wild and Russian Mountain Ash, produces fruits with one of the highest known content of hydrophilic antioxidants. Aronia fruit contains a potent blend of anthocyanins, polyphenols, and flavonoids. The most popular way of consuming the fruit is through juicing. Yet, due to very high concentrations of tannins in the juice, very few food-related applications have been developed. Resin extraction of antioxidants provides an alternative for utilizing valuable phytochemicals from crops for applications in the food industry as nutraceutical supplements and more. To increase the market value of the plant, it is important to determine what resins can extract the optimum concentration of antioxidants from aronia juice, pulp, and whole berries. We have shown that macroporous resins such as Amberlite XAD 1180N, Amberlite XAD 7HP, Amberlite XAD 761, and Amberlite FPX66, which have been reported to be effective in extracting the anthocyanins and polyphenols from other fruit juices, skins of red grapes, and the wild breed, are also effective for use in juice, pulp, and whole fruits of . However, the extremely high content of antioxidants presents a challenge to obtaining high recovery; a notable change in the juice/resin ratio is required to obtain a higher recovery value. Our results showed that Amberlite FPX66 was the best at extracting anthocyanins, polyphenols, and flavonoids from aronia juice. A separate experiment conducted to determine how to optimize the efficiency of FPX66 extraction revealed that increasing the resin/juice ratio increased the percent recovery of anthocyanins from aronia juice. Moreover, we have compared recovery between juice, pulp, and whole aronia berries and batch versus column extraction.
抗氧化剂在人体中起着至关重要的作用，它能保护细胞免受自由基（氧化反应的高活性产物）造成的损伤。抗氧化剂的一个主要来源是水果和蔬菜。黑果腺肋花楸是19世纪末通过将野生花楸和俄罗斯花楸杂交培育出的品种，其果实中亲水性抗氧化剂的含量是已知最高的之一。黑果腺肋花楸果实含有花青素、多酚和黄酮类化合物的有效混合物。食用这种水果最常见的方式是榨汁。然而，由于果汁中单宁含量非常高，很少有与食品相关的应用被开发出来。从树脂中提取抗氧化剂为利用作物中有价值的植物化学物质在食品工业中用作营养补充剂等应用提供了一种替代方法。为了提高这种植物的市场价值，确定哪些树脂能从黑果腺肋花楸果汁、果肉和整个浆果中提取出最佳浓度的抗氧化剂很重要。我们已经表明，大孔树脂如Amberlite XAD 1180N、Amberlite XAD 7HP、Amberlite XAD 761和Amberlite FPX66，据报道它们在从其他果汁、红葡萄皮和野生品种中提取花青素和多酚方面有效，在黑果腺肋花楸的果汁、果肉和整个果实中使用也有效。然而，抗氧化剂的极高含量对获得高回收率提出了挑战；需要显著改变果汁/树脂比例才能获得更高的回收率值。我们的结果表明，Amberlite FPX66在从黑果腺肋花楸果汁中提取花青素、多酚和黄酮类化合物方面效果最佳。为确定如何优化FPX66提取效率而进行的一项单独实验表明，增加树脂/果汁比例可提高黑果腺肋花楸果汁中花青素的回收率百分比。此外，我们还比较了果汁、果肉和整个黑果腺肋花楸浆果之间的回收率以及分批提取和柱式提取之间的回收率。
