Mao Chao, Lei Guang, Horbath Amber, Gan Boyi
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States.
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States; The University of Texas MD Anderson UTHealth Graduate School of Biomedical Sciences, Houston, TX, United States.
Methods Cell Biol. 2022;172:37-50. doi: 10.1016/bs.mcb.2022.05.003. Epub 2022 Jun 25.
Lipid peroxidation occurs under conditions where reactive oxygen species (ROS) readily react with vulnerable lipids on cell membranes. Polyunsaturated fatty acids (PUFAs) are highly susceptible to lipid peroxidation because of their unstable double bonds. Because the cell membrane is particularly rich in PUFAs, it is often the site at which many lipid peroxidation chain reactions occur. Lipid peroxidation is considered the ultimate trigger of ferroptosis, an iron-dependent form of non-apoptotic cell death. Radiotherapy is a common cancer treatment that uses high-energy ionizing radiation to kill cancer cells, and radiation-induced cell death is partially attributed to lipid peroxidation-driven ferroptosis. Here, we describe methods to assess lipid peroxidation in irradiated cells. The same techniques can be applied to a variety of lipid peroxidation measurements under different treatment conditions.
脂质过氧化作用发生在活性氧（ROS）容易与细胞膜上易氧化的脂质发生反应的条件下。多不饱和脂肪酸（PUFA）因其不稳定的双键而极易发生脂质过氧化作用。由于细胞膜中富含PUFA，所以这里常常是许多脂质过氧化链式反应发生的场所。脂质过氧化作用被认为是铁死亡（一种铁依赖性非凋亡性细胞死亡形式）的最终触发因素。放射治疗是一种常见的癌症治疗方法，它利用高能电离辐射杀死癌细胞，而辐射诱导的细胞死亡部分归因于脂质过氧化作用驱动的铁死亡。在此，我们描述了评估受辐照细胞中脂质过氧化作用的方法。相同的技术可应用于不同处理条件下的各种脂质过氧化作用测量。
