Fonseca Gustavo Graciano
Faculty of Natural Resource Sciences, School of Business and Science, University of Akureyri, Borgir v. Nordurslod, 600 Akureyri, Iceland.
3 Biotech. 2022 Oct;12(10):259. doi: 10.1007/s13205-022-03324-x. Epub 2022 Sep 3.
ATCC 26,548 was cultivated in aerobic chemostats with [1-C] and [U-C] glucose as carbon source under three different growth conditions (0.10, 0.25, and 0.5 h) to evaluate metabolic fluxes. Carbon balances closed always within 97-102%. Growth was carbon limited, and the cell yield on glucose was the same. The extracellular side-product formation was very low, totaling 0.0008 C-mol C-mol substrate at 0.5 h. The intracellular flux ratios did not show significant variation for metabolic flux analysis from labelling and biomass composition and metabolic flux ratio analysis from labelling. The observed strictly oxidative metabolism and the stability of the metabolism in terms of fluxes even at high growth rates, without triggering out the synthesis of by-products, is an extremely desired condition that underlines the potential of for biotechnological biomass-related applications and the comprehension of the metabolic pools and pathways is an important step to engineering this organism.
The online version contains supplementary material available at 10.1007/s13205-022-03324-x.
将ATCC 26548在好氧恒化器中培养，以[1-C]和[U-C]葡萄糖作为碳源，在三种不同生长条件下（0.10、0.25和0.5小时）评估代谢通量。碳平衡始终在97%-102%范围内闭合。生长受碳限制，葡萄糖的细胞产率相同。细胞外副产物形成非常低，在0.5小时时总计为0.0008 C-摩尔/摩尔底物。对于来自标记和生物质组成的代谢通量分析以及来自标记的代谢通量比分析，细胞内通量比未显示出显著变化。观察到的严格氧化代谢以及即使在高生长速率下代谢通量方面的稳定性，且不引发副产物的合成，是一种极其理想的条件，突出了其在生物技术生物质相关应用中的潜力，而理解代谢库和途径是对该生物体进行工程改造的重要一步。
在线版本包含可在10.1007/s13205-022-03324-x获取的补充材料。
