Heparin-like activity of insoluble sulphonated polystyrene resins. Part III: Binding of dicarboxylic amino acids.

It has been demonstrated previously that polystyrene sulphonate possesses anticoagulant properties and that the binding of some amino acids could enhance the heparin-like properties of such resins. These properties depend on the surface density of the active groups, the nature and binding of the group and on the net change borne by the polymer. In this paper, we describe the preparation of copolystyrene (sulphonate-dicarboxylic amino acid sulphamide) resins. By measuring their antithrombotic-surface-activity, we demonstrate that the activity developed by each carboxyl group is at least roughly the same as the activity of one sulphonate group, except in the case of aspartic acid sulphamide resin for which a cooperative effect is shown. The anticoagulant properties of resins bearing phosphonate or monocarboxylic amino acid sulphamides are also examined.