Laboratory of Genetics, Biotechnology and Valorization of Bioresources, University of Biskra, BP 145RP, Biskra 07000, Algeria.
Laboratory of Characterization and Valorization of Natural Resources, University Mohamed El Bachir El Ibrahimi, Bordj Bou-Arreridj 34000, Algeria.
Food Res Int. 2022 Oct;160:111714. doi: 10.1016/j.foodres.2022.111714. Epub 2022 Jul 30.
The pharmaceutical and nutraceutical industries benefit greatly from recycling and transforming non-utilized parts of medicinal plants from agro-industrial operations into value added products. Hence, the aim of this work was to study the potential nutraceutical and pharmaceutical applications of Bunium ferulaceum Sm. aerial parts, in order to maximize their value. The phenolic profile of their hydromethanolic extract was determined and its antioxidant activity was evaluated in vitro and in vivo alongside with its anti-inflammatory activity and safety profile. The extract exerted an in vitro antioxidant activity mainly through radical scavenging (DPPH IC: 14.0 ± 0.3 µg/ml) and iron chelating ability (24 ± 2 µg/ml), while, in vivo, the extract did not cause any mortality or visible signs of acute toxicity at high dose (2000 mg/kg body weight). The supplementation of the extract at different doses improved mice liver redox state by increasing catalase and reduced glutathione levels and reducing lipid peroxidation, without causing any toxicity. Moreover, the extract efficiently inhibited xylene induced ear inflammation (62 %). These different bioactivities were linked to the phenolic compounds present in the extract, particularly, chlorogenic acid (78 ± 6 mg/g extract), rutin (44 ± 2 mg/g extract) and hesperidin (56 ± 9 mg/g extract). However, further studies should be carried out on the isolated major compounds found in the extract to correlate the activity with these compounds or their mixture. The wasted aerial parts of Bunium ferulaceum Sm. proved to be a valuable source of polyphenols and exhibited interesting health promoting effects with no toxicity. Thus, Bunium ferulaceum Sm. aerial parts can be included in nutraceutical formulations or used as functional food and the extracted compounds may be used as an alternative food preservative.
制药和营养保健品行业从农业产业操作中回收和转化药用植物的未利用部分为增值产品中受益匪浅。因此，本工作的目的是研究 Bunium ferulaceum Sm. 地上部分的潜在营养保健品和药物应用，以最大限度地提高其价值。测定了其水-甲醇提取物的酚类成分，并评估了其体外和体内的抗氧化活性、抗炎活性和安全性。该提取物在体外主要通过自由基清除（DPPH IC：14.0 ± 0.3 µg/ml）和铁螯合能力（24 ± 2 µg/ml）发挥抗氧化活性，而在体内，高剂量（2000 mg/kg 体重）时提取物不会引起任何死亡率或明显的急性毒性迹象。以不同剂量补充提取物可通过增加过氧化氢酶和还原型谷胱甘肽水平并减少脂质过氧化来改善小鼠肝脏的氧化还原状态，而不会引起任何毒性。此外，提取物有效地抑制了二甲苯诱导的耳炎症（62%）。这些不同的生物活性与提取物中存在的酚类化合物有关，特别是绿原酸（78 ± 6 mg/g 提取物）、芦丁（44 ± 2 mg/g 提取物）和橙皮苷（56 ± 9 mg/g 提取物）。然而，应该对从提取物中分离出的主要化合物进行进一步研究，以将活性与这些化合物或其混合物相关联。Bunium ferulaceum Sm. 的废弃地上部分被证明是多酚的宝贵来源，并具有有趣的促进健康的作用而没有毒性。因此，Bunium ferulaceum Sm. 地上部分可以包含在营养保健品配方中或用作功能性食品，并且提取的化合物可以用作替代食品防腐剂。
