Comparative Transcriptomic Analyses Reveal the Regulatory Mechanism of Nutrient Limitation-Induced Sporulation of in Submerged Fermentation.

is a precious edible and medicinal mushroom with various biological activities, such as hepatoprotection, antitumor, antivirus, immunoregulation, and intestinal flora regulation. However, the wild fruiting bodies of are scarce and expensive. Submerged fermentation based on spore inoculation has become the most efficient and popular artificial culture method for . In order to complement the mechanism of asexual sporulation of in submerged fermentation, and provide a theoretical basis to further improve the sporulation, comparative transcriptomics analysis using RNA-seq and RT-qPCR were conducted on mycelia cultured under different nutritional conditions to reveal the regulatory mechanism underlying the asexual sporulation induced by nutrient limitation. The obtained mechanism is as follows: under nitrogen starvation, the corresponding sensors transmit signals to genes, such as and , and promote their expression. Among these genes, AreA has a direct or indirect effect on and promotes its expression, further enhancing the expression of . Meanwhile, TmpA has a direct or indirect effect on and promotes its expression; under carbon starvation, transport protein Rco-3, as a glucose sensor, directly or indirectly transmits signals to and promotes its expression. BrlA promotes the expression of gene, which further enhances the expression of gene, and then directly leads to asexual sporulation and promotes spore maturation; meanwhile, can also promote cell autolysis, which provides energy and raw materials for sporulation.