State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.
Int J Mol Sci. 2022 Aug 24;23(17):9575. doi: 10.3390/ijms23179575.
Meat production performance is one of the most important factors in determining the economic value of poultry. Myofiber is the basic unit of skeletal muscle, and its physical and chemical properties determine the meat quality of livestock and poultry to a certain extent. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha () as a transcriptional coactivator has been found to be widely involved in a series of biological processes. However, is still poorly understood in chickens. In this manuscript, we reported that was highly expressed in slow-twitch myofibers. facilitated mitochondrial biogenesis and regulated skeletal muscle metabolism by mediating the flux of glycolysis and the TCA cycle. Gain- and loss-of-function analyses revealed that promoted intramuscular fatty acid oxidation, drove the transformation of fast-twitch to slow-twitch myofibers, and increased chicken skeletal muscle mass. Mechanistically, the expression level of is regulated by . Our findings help to understand the genetic regulation of skeletal muscle development and provide a molecular basis for further research on the antagonism of skeletal muscle development and fat deposition in chickens.
肉用生产性能是决定家禽经济价值的最重要因素之一。肌纤维是骨骼肌的基本单位，其理化性质在一定程度上决定了畜禽的肉质。过氧化物酶体增殖物激活受体γ共激活因子 1-α（）作为一种转录共激活因子，已被发现广泛参与一系列生物学过程。然而，在鸡中，其仍然知之甚少。在本手稿中，我们报道 在慢肌纤维中高度表达。 通过介导糖酵解和 TCA 循环的通量，促进线粒体生物发生并调节骨骼肌代谢。获得和丧失功能分析表明， 促进肌内脂肪酸氧化，驱动快肌向慢肌纤维的转化，并增加鸡的骨骼肌质量。在机制上， 的表达水平受 调节。我们的研究结果有助于了解骨骼肌发育的遗传调控，并为进一步研究鸡骨骼肌发育和脂肪沉积的拮抗作用提供了分子基础。
