Ma Manting, Cai Bolin, Jiang Liang, Abdalla Bahareldin Ali, Li Zhenhui, Nie Qinghua, Zhang Xiquan
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.
Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, Guangdong, China.
Cells. 2018 Dec 4;7(12):243. doi: 10.3390/cells7120243.
Emerging studies indicate important roles for non-coding RNAs (ncRNAs) as essential regulators in myogenesis, but relatively less is known about their function. In our previous study, we found that lncRNA-Six1 can regulate in to participate in myogenesis. Here, we studied a microRNA (miRNA) that is specifically expressed in chickens (miR-1611). Interestingly, miR-1611 was found to contain potential binding sites for both lncRNA-Six1 and , and it can interact with lncRNA-Six1 to regulate expression. Overexpression of miR-1611 represses the proliferation and differentiation of myoblasts. Moreover, miR-1611 is highly expressed in slow-twitch fibers, and it drives the transformation of fast-twitch muscle fibers to slow-twitch muscle fibers. Together, these data demonstrate that miR-1611 can mediate the regulation of Six1 by lncRNA-Six1, thereby affecting proliferation and differentiation of myoblasts and transformation of muscle fiber types.
新兴研究表明，非编码RNA（ncRNAs）在肌生成过程中作为重要调节因子发挥着关键作用，但其功能相对鲜为人知。在我们之前的研究中，我们发现lncRNA-Six1可以调节参与肌生成。在此，我们研究了一种在鸡中特异性表达的微小RNA（miRNA，即miR-1611）。有趣的是，发现miR-1611同时含有lncRNA-Six1和[此处原文缺失相关基因名称]的潜在结合位点，并且它可以与lncRNA-Six1相互作用来调节[此处原文缺失相关基因名称]的表达。miR-1611的过表达会抑制成肌细胞的增殖和分化。此外，miR-1611在慢肌纤维中高表达，并且它促使快肌纤维向慢肌纤维转变。总之，这些数据表明miR-1611可以介导lncRNA-Six1对Six1的调节，从而影响成肌细胞的增殖和分化以及肌纤维类型的转变。
