Research Center for Studies of Food Quality and Agricultural Products, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59, Mărăști Bd., 011464 Bucharest, Romania.
Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59, Mărăști Bd., 011464 Bucharest, Romania.
Int J Mol Sci. 2022 Aug 28;23(17):9750. doi: 10.3390/ijms23179750.
Genetic variability is extremely important, not only for the species' adaptation to environmental challenges, but also for the creation of novel varieties through plant breeding. Tomato is an important vegetable crop, as well as a model species in numerous genomic studies. Its genome was fully sequenced in 2012 for the 'Heinz 1706' variety, and since then, resequencing efforts have revealed genetic variability data that can be used for multiple purposes, including triggering mechanisms of biotic and abiotic stress resistance. The present study focused on the analysis of the genome variation for eight Romanian local tomato varieties using next-generation sequencing technique, and as a case study, the sequence analysis of the and loci, to determine which genotypes might be good candidates for future breeding of tomato varieties resistant to species. The analysis of the locus identified several genotypes that could be donors of the gene conferring resistance to race 1. Sequencing for the first time Romanian genotypes enriched the existing data on various world tomato genetic resources, but also opened the way for the molecular breeding in Romania. Plant breeders can use these data to create novel tomato varieties adapted to the ever-changing environment.
遗传变异非常重要，不仅对于物种适应环境挑战，而且对于通过植物育种创造新的品种也非常重要。番茄是一种重要的蔬菜作物，也是许多基因组研究的模式物种。2012 年，“Heinz 1706”品种的基因组被完全测序，此后，重测序工作揭示了遗传变异数据，可用于多种用途，包括触发生物和非生物胁迫抗性的机制。本研究利用下一代测序技术，对 8 个罗马尼亚本地番茄品种的基因组变异进行了分析，并以 和 位点的序列分析为例，确定哪些基因型可能是未来培育抗 物种番茄品种的良好候选者。对 位点的分析确定了几个可能是抗 1 号小种的 基因供体的基因型。首次对罗马尼亚基因型进行测序，丰富了各种世界番茄遗传资源的数据，也为罗马尼亚的分子育种开辟了道路。植物育种者可以利用这些数据来创造适应不断变化的环境的新型番茄品种。
