Kibret Meseret Ewunetu, Terfasa Tatek Temesgen, Alemea Melakuu Tesfaye
Department of Chemical Engineering, Adama Science and Technology University, Adama, Ethiopia.
Heliyon. 2022 Aug 28;8(8):e10346. doi: 10.1016/j.heliyon.2022.e10346. eCollection 2022 Aug.
Elastin and collagen were isolated from waste broil skin and modified with l-lactic acid oligomer for the efficacy of substituting petroleum based ointment base matrix. Paraffin wax, which is one of the most extensively used petroleum ointment derivative is well known for its incompatibility with the skin. Chronically it clogs the skin pores, which subsequently affects the release of moisture. To mitigate this problem, a novel approach has been followed to synthesize a fully green and biocompatible ointment base matrix. The extracted Elastin and Collagen (which exists naturally in our skin) and lactic acid monomer (commonly known as a part of a body fluid) mixed at different proportion and used to synthesize a lactic acid modified elastin/collagen (OLLA-m-ELN/COL) bioconjugate matrix with lubricating characteristics via in-situ polycondensation reaction. The macromolecular interaction between Amide I group of elastin/collagen and C=O of a lactic acid oligomer was confirmed by FTIR analysis. Organoleptic analysis, spreadability, pH, and viscosity were analyzed. The six-mass loss stages which was observed in the ELN/COL matrix was changed into a single mass loss for the synthesized bioconjugate with improved thermal stability. The thermal stability improvement can be correlated with the formation of secondary macromolecular interaction.
从废弃的烤鸡皮中分离出弹性蛋白和胶原蛋白，并用L-乳酸低聚物进行改性，以替代石油基软膏基质。石蜡是使用最广泛的石油软膏衍生物之一，因其与皮肤不相容而闻名。长期使用会堵塞毛孔，进而影响皮肤水分的释放。为缓解这一问题，采用了一种新方法来合成完全绿色且生物相容的软膏基质。将提取的弹性蛋白和胶原蛋白（天然存在于我们的皮肤中）以及乳酸单体（通常作为体液的一部分为人所知）按不同比例混合，通过原位缩聚反应合成具有润滑特性的乳酸改性弹性蛋白/胶原蛋白（OLLA-m-ELN/COL）生物共轭基质。通过傅里叶变换红外光谱（FTIR）分析证实了弹性蛋白/胶原蛋白的酰胺I基团与乳酸低聚物的C=O之间的大分子相互作用。对其进行了感官分析、铺展性、pH值和粘度分析。在ELN/COL基质中观察到的六个质量损失阶段，在合成的生物共轭物中转变为单一质量损失，且热稳定性得到改善。热稳定性的提高与二级大分子相互作用的形成有关。
