Department of Chemistry and Biochemistry, Queens College-CUNY, Flushing, New York 11367, United States.
The Graduate Center of CUNY, New York, New York 10016, United States.
Anal Chem. 2022 Sep 20;94(37):12614-12620. doi: 10.1021/acs.analchem.2c01216. Epub 2022 Sep 9.
Extracellular vesicles (EVs) released from biological cells have attracted considerable interest due to their potential for cancer diagnostics and important role in cell signaling. Most previously reported studies have been concerned with the detection of EVs in biofluids and analysis of proteins and nucleic acids they contain. Electrochemical resistive-pulse (ERP) sensing enables direct detection of single EVs released from a specific cell and analysis of reactive oxygen and nitrogen species in such vesicles. Here, we demonstrate the applicability of ERP sensing to distinguish between nontransformed and cancerous breast cell lines as well as between breast cancer cell lines with different metastatic potential. Another application of ERP sensing is in real-time monitoring of changes in a single cell induced by a chemical agent. This approach is potentially useful for evaluating the efficacy of therapeutic agents, including those that trigger breast cancer cell death by inducing intense oxidative stress.
细胞外囊泡（EVs）从生物细胞中释放出来，由于其在癌症诊断中的潜力以及在细胞信号转导中的重要作用，引起了相当大的关注。以前的大多数研究都集中在生物体液中 EVs 的检测以及分析它们所包含的蛋白质和核酸上。电化学电阻脉冲（ERP）传感可直接检测从特定细胞释放的单个 EV，并分析此类囊泡中的活性氧和氮物种。在这里，我们证明了 ERP 传感在区分非转化和癌变乳腺细胞系以及具有不同转移潜力的乳腺癌细胞系方面的适用性。ERP 传感的另一个应用是实时监测单个细胞在化学试剂作用下发生的变化。这种方法对于评估治疗剂的疗效可能是有用的，包括通过诱导强烈的氧化应激来触发乳腺癌细胞死亡的治疗剂。
