血根碱通过抑制 mTOR 信号通路抑制鼻咽癌细胞增殖、迁移和侵袭。
Sanguinarine suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma inhibiting mTOR signaling.
机构信息
School of Basic Medical Sciences, Guangxi Medical University, Nanning 530021, China.
Institute of Biomedical Research, Yunnan University, Kunming 650500, China.
出版信息
J Tradit Chin Med. 2022 Oct;42(5):687-692. doi: 10.19852/j.cnki.jtcm.20220426.001.
OBJECTIVE
To confirm the anti-NPC effect of sanguinarine (SA) through a series of wet experiments.
METHODS
NPC cell viability was determined by proliferation experiment. Cell clone formation experiment, cell scratch test, transwell migration and invasion experiment and flow cytometry-based cell apoptosis assay were further performed. In addition, Western blotting was performed to investigate the cell signaling pathway. All the relevant experimental data were statistically processed using SPSS 16.0 software.
RESULTS
The results showed that sanguinarine represented a time and dose dependent inhibition effects on NPC cell proliferation including the low differentiated CNE2 cells and high metastatic 5-8F cells, along with the cell cloning ability reduction. In addition, sanguinarine has a certain inhibitory effect on the invasion and migration of NPC cells. Mechanistically, sanguinarine displayed the anti-NPC effects mainly involved into the suppression of mTOR signaling and cell apoptosis, which is closely associated with the tumor growth and metastatic malignancy.
CONCLUSIONS
Collectively, we discover that sanguinarine is a new high-efficiency anti-NPC monomer of Chinese medicine, with a value for the follow-up pre-clinical research.
目的
通过一系列湿实验证实血根碱（SA）的抗 NPC 作用。
方法
增殖实验测定 NPC 细胞活力。进一步进行细胞克隆形成实验、细胞划痕实验、Transwell 迁移和侵袭实验以及基于流式细胞术的细胞凋亡检测。此外，通过 Western blot 检测细胞信号通路。使用 SPSS 16.0 软件对所有相关实验数据进行统计处理。
结果
结果表明，血根碱对 NPC 细胞增殖具有时间和剂量依赖性抑制作用，包括低分化 CNE2 细胞和高转移性 5-8F 细胞，同时降低细胞克隆能力。此外，血根碱对 NPC 细胞的侵袭和迁移具有一定的抑制作用。从机制上讲，血根碱发挥抗 NPC 作用主要涉及抑制 mTOR 信号和细胞凋亡，这与肿瘤生长和转移恶性密切相关。
结论
综上所述，我们发现血根碱是一种新型高效的中药抗 NPC 单体，具有后续临床前研究的价值。
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