Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Molecular and Cellular Biology Program, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Nano Lett. 2022 Sep 28;22(18):7579-7587. doi: 10.1021/acs.nanolett.2c02605. Epub 2022 Sep 9.
The cell membrane is a complex mixture of lipids, proteins, and other components. By forming dynamic lipid domains, different membrane molecules can selectively interact with each other to control cell signaling. Herein, we report several new types of lipid-DNA conjugates, termed as "DNA zippers", which can be used to measure cell membrane dynamic interactions and the formation of lipid domains. Dependent on the choice of lipid moieties, cholesterol- and sphingomyelin-conjugated DNA zippers specifically locate in and detect membrane lipid-ordered domains, while in contrast, a tocopherol-DNA zipper can be applied for the selective imaging of lipid-disordered phases. These versatile and programmable probes can be further engineered into membrane competition assays to simultaneously detect multiple types of membrane dynamic interactions. These DNA zipper probes can be broadly used to study the correlation between lipid domains and various cellular processes, such as the epithelial-mesenchymal transition.
细胞膜是一种复杂的脂质、蛋白质和其他成分的混合物。通过形成动态脂质域，不同的膜分子可以选择性地相互作用，以控制细胞信号转导。在此，我们报告了几种新型的脂质-DNA 缀合物，称为“DNA 拉链”，可用于测量细胞膜动态相互作用和脂质域的形成。根据脂质部分的选择，胆固醇和神经鞘磷脂缀合的 DNA 拉链特异性定位并检测膜脂质有序域，而相反，生育酚-DNA 拉链可用于选择性成像脂质无序相。这些多功能和可编程的探针可进一步设计成膜竞争测定法，以同时检测多种类型的膜动态相互作用。这些 DNA 拉链探针可广泛用于研究脂质域与各种细胞过程之间的相关性，例如上皮-间充质转化。
