Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, United States.
Department of Plant Pathology, North Dakota State University, Fargo, ND 58108, United States.
Food Chem. 2023 Jan 30;400:134016. doi: 10.1016/j.foodchem.2022.134016. Epub 2022 Aug 27.
This work aims to investigate antifungal, mycotoxin inhibitory efficacy of the hop essential oil (HEO) nanoemulsion and their mode of action (MOA) against Fusarium graminearum isolate, a fungal pathogen causing Fusarium Head Blight (FHB) in cereal crops. The HEO, primarily consisting of terpenes and terpenoids, was encapsulated in nanoemulsion droplets. Physically stable HEO-in-water nanoemulsion was fabricated using 0.5 wt% of tween 80 and 5 wt% oil phase comprising 30 % of Ostwald ripening inhibitor and 70 % of HEO. In terms of antifungal effect, HEO nanoemulsion could not only effectively inhibit mycelial growth and spore germination of F. graminearum isolates, but also remarkably suppress the production of deoxynivalenol (DON) and its derivatives in rice culture by applying 750 μg of HEO/g rice. Our studies on the MOA showed that HEO nanoemulsion could alter the contents of total lipid and chitin in outer cell membrane as well as damaging cytoplasmic membrane.
本研究旨在探究啤酒花精油（HEO）纳米乳液的抗真菌、抑制霉菌毒素的功效及其对禾谷镰刀菌（一种引起谷物赤霉病（FHB）的真菌病原体）的作用模式。HEO 主要由萜烯和萜类化合物组成，被包裹在纳米乳液液滴中。使用 0.5wt%的吐温 80 和 5wt%的油相（包括 30%的奥斯特瓦尔德熟化抑制剂和 70%的 HEO）制备出物理稳定的 HEO-水纳米乳液。在抗真菌效果方面，HEO 纳米乳液不仅能有效抑制禾谷镰刀菌分离株的菌丝生长和孢子萌发，还能通过在 750μg HEO/g 大米上应用显著抑制大米培养物中脱氧雪腐镰刀菌烯醇（DON）及其衍生物的产生。我们对作用模式的研究表明，HEO 纳米乳液可以改变细胞膜外总脂质和几丁质的含量，并破坏细胞质膜。
