Key Laboratory for Metabolic Diseases in Chinese Medicine, First College of Clinical Medicine, Nanjing University of Chinese Medicine, China; Department of Physiology, Hanlin College, Nanjing University of Chinese Medicine, China.
Department of Endocrinology, Clinical Medical College, Yangzhou University, China.
Physiol Behav. 2022 Nov 1;256:113958. doi: 10.1016/j.physbeh.2022.113958. Epub 2022 Sep 7.
Exercise training improves obesity-induced metabolic diseases through regulation of adipokines. Previous studies have shown that adipocyte-spexin participates in metabolic diseases such as obesity and diabetes via the modulation of energy homeostasis and insulin resistance. The objective of this research was to investigate the effects of swimming exercise on the levels of adipocyte-spexin and the underlying mechanisms. The normal chow diet (NC)-fed and high-fat diet (HFD)-fed mice were divided into exercise or sedentary groups. The expression and secretion of spexin in adipose tissue were assessed by quantitative real-time PCR and ELISA. The present findings uncovered the effect of exercise-induced spexin expression in the adipose tissue of obese mice. Besides, chronic exercise-induced upregulation of adipose spexin may be mediated by COUP-TF2 and KLF9. In addition, constant-moderate intensity exercise increased the levels of GLUT4, SIRT1 and PGC-1α in the skeletal muscles of mice. These results suggest that spexin is a potential mediator for exercise to ameliorate obesity-induced insulin resistance, namely, the beneficial effect of exercise on insulin sensitivity is at least partly mediated by spexin. Thus, exercise restores spexin production and release, which increases insulin sensitivity and maintains metabolic balance in the adipose tissues of HFD-induced obese mice.
运动训练通过调节脂肪因子改善肥胖引起的代谢性疾病。先前的研究表明，脂肪细胞分泌素通过调节能量平衡和胰岛素抵抗参与肥胖和糖尿病等代谢性疾病。本研究旨在探讨游泳运动对脂肪细胞分泌素水平的影响及其潜在机制。正常饲料喂养（NC）和高脂肪饲料喂养（HFD）的小鼠被分为运动或不运动组。通过定量实时 PCR 和 ELISA 评估脂肪组织中分泌素的表达和分泌。本研究揭示了运动诱导肥胖小鼠脂肪组织中分泌素表达的作用。此外，慢性运动诱导脂肪分泌素的上调可能是由 COUP-TF2 和 KLF9 介导的。此外，恒等-中等强度运动增加了小鼠骨骼肌中 GLUT4、SIRT1 和 PGC-1α 的水平。这些结果表明，分泌素可能是运动改善肥胖引起的胰岛素抵抗的潜在介质，即运动对胰岛素敏感性的有益作用至少部分是通过分泌素来介导的。因此，运动恢复了分泌素的产生和释放，增加了胰岛素敏感性，并维持了 HFD 诱导肥胖小鼠脂肪组织中的代谢平衡。
