Zhang Xu, Li Hang-Yuan, Shao Jian-Wei, Pei Ming-Chao, Cao Chong, Huang Fu-Qiang, Sun Ming-Fei
School of Life Science and Engineering, Foshan University, Foshan, China.
Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, China.
Front Microbiol. 2022 Aug 25;13:977405. doi: 10.3389/fmicb.2022.977405. eCollection 2022.
The increasing prevalence and transmission of tick-borne diseases, especially those emerging ones, have posed a significant threat to public health. Thus, the discovery of neglected pathogenic agents carried and transmitted by ticks is urgently needed. Using unbiased high-throughput sequencing, a novel Orthonairovirus designated as Meihua Mountain virus (MHMV), was identified in bloodsucking ticks collected from cattle and wild boars in Fujian province, Southeastern China. The full-length genome was determined by RT-PCR and RACE. Genomic architecture of MHMV shares typical features with orthonairoviruses. Phylogenetic analyses suggested that MHMV is clustered into the Nairobi sheep disease (NSD) genogroup of the genus and is closely related to the Hazara virus. The RdRp, GPC, and N protein of MHMV shares 62.3%-83.5%, 37.1%-66.1%, and 53.4%-77.3% amino acid identity with other NSD genogroup viruses, respectively, representing a novel species. The overall pooled prevalence of MHMV in ticks was 2.53% (95% CI: 1.62%-3.73%, 22 positives of 134 tick pools), with 7.38% (95% CI: 3.84%-12.59%, 11 positives of 18 pools) in , 6.02% (95% CI: 1.85%-14.22%, four positives of eight pools) in , 25.03% (95% CI: 9.23%-54.59%, six positive of eight pools) in , and 0.16% (95% CI: 0.01%-0.72%, one positive of 100 pools) in . This study presented the first report of tick-carried Orthonairovirus in Fujian province and highlighted the broad geographic distribution and high genetic diversity of orthonairoviruses in China.
蜱传疾病，尤其是新出现的蜱传疾病的发病率和传播率不断上升，已对公众健康构成重大威胁。因此，迫切需要发现蜱虫携带和传播的被忽视的病原体。利用无偏向高通量测序技术，在中国东南部福建省采集的吸血蜱虫中鉴定出一种新型正内罗病毒，命名为梅花山病毒（MHMV）。通过RT-PCR和RACE确定了其全长基因组。MHMV的基因组结构与正内罗病毒具有典型特征。系统发育分析表明，MHMV聚类到该属的内罗毕羊病（NSD）基因组群中，与哈扎拉病毒密切相关。MHMV的RdRp、GPC和N蛋白与其他NSD基因组群病毒的氨基酸同一性分别为62.3%-83.5%、37.1%-66.1%和53.4%-77.3%，代表一个新物种。蜱虫中MHMV的总体合并流行率为2.53%（95%CI：1.62%-3.73%，134个蜱虫池中有22个阳性），其中[此处原文缺失蜱虫种类信息]为7.38%（95%CI：3.84%-12.59%，18个池中有11个阳性），[此处原文缺失蜱虫种类信息]为6.02%（95%CI：1.85%-14.22%，8个池中有4个阳性），[此处原文缺失蜱虫种类信息]为25.03%（95%CI：9.23%-54.59%，8个池中有6个阳性），[此处原文缺失蜱虫种类信息]为0.16%（95%CI：0.01%-0.72%，100个池中有1个阳性）。本研究首次报道了福建省蜱虫携带的正内罗病毒，并强调了中国正内罗病毒广泛的地理分布和高度的遗传多样性。
