Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal Do Rio Grande Do Sul (UFRGS), Porto Alegre, RS, Brazil.
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil.
Trop Anim Health Prod. 2022 Sep 13;54(5):293. doi: 10.1007/s11250-022-03282-9.
Porcine circovirus type 3 (PCV3) has been widely detected worldwide in healthy and sick pigs. Recently its association with clinical disease and reproductive failure has been proven through the detection of intralesional viral mRNA in affected pigs. This study aims to describe the occurrence of PCV3-associated reproductive failure (abortions) in sow herds in southern Brazil. Eleven fetuses from five different litters from two herds were analyzed. These herds reported an increase in the rate of late-gestation abortions, stillbirths, and the percentage of mummified piglets. At gross examination, six of the fetuses had large caudally rotated ears and one fetus was mummified. Microscopically, multisystemic vasculitis, lymphocytic interstitial pneumonia, myocarditis, and encephalitis were observed. These six fetuses with gross and histological lesions were positive in qPCR analysis for PCV3, and PCV3 transcription was shown through in situ hybridization (ISH-RNA) within the histologic lesions. Samples from all 11 fetuses tested negative in PCR exam for Porcine Circovirus type 1 and 2, Porcine Reproductive and Respiratory Syndrome, Porcine Parvovirus, and Atypical Porcine Pestivirus. Furthermore, based on the ORF2 analysis, the PCV3a clade was identified. This is the first report of PCV3a-associated reproductive failure in pig herds in South America.
猪圆环病毒 3 型（PCV3）已在全球范围内的健康和患病猪中广泛检测到。最近，通过检测受影响猪的病灶内病毒 mRNA，已证明其与临床疾病和繁殖失败有关。本研究旨在描述巴西南部猪群中与 PCV3 相关的繁殖失败（流产）的发生情况。分析了来自两个牛群的五个不同窝的 11 个胎儿。这些牛群报告说，晚期流产、死产和木乃伊化仔猪的比例增加。大体检查时，6 个胎儿的耳朵尾部大而旋转，1 个胎儿木乃伊化。显微镜下观察到多系统血管炎、淋巴细胞性间质性肺炎、心肌炎和脑炎。这 6 个具有大体和组织学病变的胎儿在 qPCR 分析中对 PCV3 呈阳性，并且通过组织学病变内的原位杂交（ISH-RNA）显示 PCV3 转录。11 个胎儿的所有样本在 PCR 检测中均为 Porcine Circovirus 1 和 2、Porcine Reproductive and Respiratory Syndrome、Porcine Parvovirus 和 Atypical Porcine Pestivirus 均为阴性。此外，根据 ORF2 分析，确定了 PCV3a 分支。这是在南美洲猪群中首次报告与 PCV3a 相关的繁殖失败。
