Shen Jiazhi, Wang Shuangshuang, Sun Litao, Wang Yu, Fan Kai, Li Chen, Wang Hui, Bi Caihong, Zhang Fen, Ding Zhaotang
Tea Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
Tea Research Institute, Qingdao Agricultural University, Qingdao, China.
Front Plant Sci. 2022 Sep 2;13:978531. doi: 10.3389/fpls.2022.978531. eCollection 2022.
Tea ( L.), as an evergreen plant, needs a humid environment. Water deficit would diminish tea yield and quality. We analyzed the dynamic changes in the metabolite and lipid profiling of tea leaves under various drought conditions and re-watering to determine the metabolic changes in tea leaves responding to drought challenges. In all, 119 metabolites showed substantial alterations in drought-stressed tea plants, including sugars and sugar alcohols, amino acids, and tricarboxylic acid cycle intermediates and lipids. We detected 29 lipids and they were classified into phosphatidylglycerol (PG), phosphatidic acid (PA), sulfoquinovosyl-diacylglycerol (SQDG), phosphatidylcholine (PC), lyso-phosphatidylcholine (LysoPC), and phosphatidylinositol (PI). The levels of sugar, sugar alcohol, and sugar precursors may change as a response to drought stress. Compared with these metabolites, the membrane lipids showed more dynamic changes in tea under drought stresses. Furthermore, metabolic recovery was only partial, with the majority of the examined metabolites exhibiting significantly different levels between samples from re-watered and well-watered tea plants. The findings also showed that comprehensive metabolomic and lipidomic approaches were efficient in elucidating the impacts of drought stress on tea plant metabolism. Our findings are valuable for understanding the mechanisms behind drought tolerance in tea plants from the metabolism perspective and utilizing the compounds to improve the drought tolerance of tea plants.
茶树（L.）作为一种常绿植物，需要湿润的环境。水分亏缺会降低茶叶产量和品质。我们分析了不同干旱条件及复水后茶树叶片代谢物和脂质谱的动态变化，以确定茶树叶片对干旱挑战的代谢变化。总共119种代谢物在干旱胁迫的茶树中表现出显著变化，包括糖类和糖醇类、氨基酸、三羧酸循环中间产物和脂质。我们检测到29种脂质，它们被分为磷脂酰甘油（PG）、磷脂酸（PA）、磺基喹喔啉二酰甘油（SQDG）、磷脂酰胆碱（PC）、溶血磷脂酰胆碱（LysoPC）和磷脂酰肌醇（PI）。糖、糖醇和糖前体的水平可能会因干旱胁迫而改变。与这些代谢物相比，膜脂在干旱胁迫下的茶树中表现出更动态的变化。此外，代谢恢复只是部分恢复，大多数检测到的代谢物在复水茶树和水分充足茶树的样本之间表现出显著不同的水平。研究结果还表明，综合代谢组学和脂质组学方法在阐明干旱胁迫对茶树代谢的影响方面是有效的。我们的研究结果对于从代谢角度理解茶树耐旱机制以及利用这些化合物提高茶树耐旱性具有重要价值。
