Jiang Chunyu, Liu Fangshen, Yang Hangao, Yang Mengyue, Li Zhanlin, Han Tong, Li Dahong, Hua Huiming
Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, PR China.
Department of Pharmaceutical Engineering, College of Life Science & Technology, Heilongjiang Bayi Agricultural University, 5 Xinfeng Road, Daqing, 163319, PR China.
Phytochemistry. 2022 Dec;204:113436. doi: 10.1016/j.phytochem.2022.113436. Epub 2022 Sep 18.
Plants of the Cephalotaxus genus are rich in structurally diverse and naturally bioactive components, while limited studies have been reported for Cephalotaxus oliveri. Two undescribed flavonolignans and four undescribed biflavonoids, as well as thirteen known compounds, were isolated from the twigs and leaves of C. oliveri. Their structures were characterized by spectroscopic data analysis, and the absolute configurations were determined by electronic circular dichroism (ECD) calculations. All the isolated compounds were assayed for their neuroprotective activity against hydrogen peroxide (HO)-induced SH-SY5Y cell injury. All six undescribed compounds were effective to some degree, and umcephabiflovin B, apigenin 5-O-α-L-rhamnopyranosyl-(1 → 2)-6″-acetyl-β-D-glucopyranoside, and apigenin 7-O-β-D-glucoside exhibited good neuroprotective activity. Umcephabiflovin B protected SH-SY5Y cells against HO-induced neurotoxicity by repressing oxidative stress and apoptosis and by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant-response element (ARE) pathway.
三尖杉属植物富含结构多样的天然生物活性成分，而关于贡山三尖杉的研究报道较少。从贡山三尖杉的嫩枝和叶子中分离出两种未描述的黄酮木脂素、四种未描述的双黄酮以及十三种已知化合物。通过光谱数据分析对其结构进行了表征，并通过电子圆二色光谱（ECD）计算确定了绝对构型。对所有分离出的化合物针对过氧化氢（HO）诱导的SH-SY5Y细胞损伤的神经保护活性进行了测定。所有六种未描述的化合物都有一定程度的效果，其中贡山双黄酮B、芹菜素5-O-α-L-鼠李糖基-(1→2)-6″-乙酰基-β-D-葡萄糖苷和芹菜素7-O-β-D-葡萄糖苷表现出良好的神经保护活性。贡山双黄酮B通过抑制氧化应激和细胞凋亡以及激活核因子红细胞2相关因子2（Nrf2）/抗氧化反应元件（ARE）途径，保护SH-SY5Y细胞免受HO诱导的神经毒性。
