Bäumert H G, Sköld S E, Kurland C G
Eur J Biochem. 1978 Sep 1;89(2):353-9. doi: 10.1111/j.1432-1033.1978.tb12536.x.
It is possible to crosslink protein to nucleic acid with diepoxybutane which reacts mainly with the N-7 of guanine as well as the thiol and aminogroups in protein; the resulting crosslinked complexes are cleavable. When this reagent is applied to ribosomes, even under conditions that minimize the extent of reaction, at least half of the ribosomal proteins can be recovered crosslinked to one or other of the major ribosomal RNA species. In addition, one fourth of the proteins can be recovered crosslinked to the RNA of the heterologous ribosomal subunit. Some of the remaining proteins may also be crosslinkable to the RNA, but additional experiments are required to confirm this.
可以用双环氧丁烷将蛋白质与核酸交联，双环氧丁烷主要与鸟嘌呤的N-7以及蛋白质中的巯基和氨基反应；所得的交联复合物是可裂解的。当将该试剂应用于核糖体时，即使在使反应程度最小化的条件下，至少一半的核糖体蛋白质可以被回收并与一种或另一种主要核糖体RNA种类交联。此外，四分之一的蛋白质可以被回收并与异源核糖体亚基的RNA交联。其余一些蛋白质也可能与RNA交联，但需要进一步的实验来证实这一点。
