de Souza Andrade Milena M, Leal Vinicius N C, Fernandes Iara G, Gozzi-Silva Sarah C, Beserra Danielle R, Oliveira Emily A, Teixeira Franciane M E, Yendo Tatiana M, Sousa Maria da Glória T, Teodoro Walcy R, Oliveira Luana de M, Alberca Ricardo W, Aoki Valéria, Duarte Alberto J S, Sato Maria N
Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo Medical School, São Paulo 05403-000, Brazil.
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.
Antioxidants (Basel). 2022 Aug 29;11(9):1690. doi: 10.3390/antiox11091690.
The formation of microthrombi in lung autopsies indicates the involvement of NETs in the immunopathogenesis of severe COVID-19. Therefore, supplements inhibiting NET formation, in association with drugs with fewer adverse effects, should be a relevant strategy to attenuate the disease. Resveratrol (RESV) is a natural polyphenol with an important antiviral and antioxidant role. To modulate neutrophils from patients infected with SARS-CoV-2, we evaluated the in vitro effect of RESV on NET formation. Herein, we investigated 190 patients hospitalized with moderate, severe, and critical symptoms at Hospital das Clínicas, Brazil. We observed that neutrophilia in patients with severe COVID-19 infection is composed of neutrophils with activated profile able to release NET spontaneously. Notably, RESV decreased the neutrophil-activated status and the release of free DNA, inhibiting NET formation even under the specific PMA stimulus. At present, there is no evidence of the role of RESV in neutrophils from patients with COVID-19 infection. These findings suggest that adjunctive therapies with RESV may help decrease the inflammation of viral or bacterial infection, improving patient outcomes.
肺尸检中微血栓的形成表明中性粒细胞胞外陷阱（NETs）参与了重症新型冠状病毒肺炎（COVID-19）的免疫发病机制。因此，联合使用不良反应较少的药物，补充抑制NET形成的物质，应是减轻该疾病的一种相关策略。白藜芦醇（RESV）是一种天然多酚，具有重要的抗病毒和抗氧化作用。为了调节感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）患者的中性粒细胞，我们评估了RESV对NET形成的体外作用。在此，我们调查了巴西临床医院收治的190例有中度、重度和危重症症状的住院患者。我们观察到，重症COVID-19感染患者的中性粒细胞增多由具有活化特征、能够自发释放NET的中性粒细胞组成。值得注意的是，RESV降低了中性粒细胞的活化状态和游离DNA的释放，即使在特定的佛波酯（PMA）刺激下也能抑制NET形成。目前，尚无证据表明RESV在COVID-19感染患者的中性粒细胞中发挥作用。这些发现表明，RESV辅助治疗可能有助于减轻病毒或细菌感染的炎症反应，改善患者预后。
