Alghamdi Ali Hendi, Khalid Asaad, Ahmed Aimun A E, Abdalgadir Haidar, Bashir Mahadi, Abdalla Ashraf N, Ashgar Sami S, Alsaid Hamdi M, Oraiby Magbool E
Surgery Department, Faculty of Medicine, Al Baha University, P. O. Box: 1998, Al Baha, 65515, Saudi Arabia.
Health Research Center, Jazan University, P. O. Box: 114, Jazan, 82511, Saudi Arabia.
Discov Oncol. 2024 Jul 15;15(1):282. doi: 10.1007/s12672-024-01138-2.
This study on Buddleja polystachya highlights its phytochemical composition, antimicrobial activity, and cytotoxic impacts. The study emphasizes the plant's potential to treat ocular diseases by identifying important compounds involved in the bioactivity through GC-MS analysis. This study explores the antimicrobial and cytotoxic potential of Buddleja polystachya (stem and leaves) extracts, with a focus on their application in treating bacterial ocular infections and their efficacy against MCF7, HT29, and HepG2 cancer cells. Through comprehensive GC-MS analysis, a diverse array of phytochemicals was identified within Buddleja polystachya stem and leaves extracts, including carbohydrates, phenolic derivatives, fatty acids, and steroidal components. The extracts were then evaluated for their biological activities, revealing significant antimicrobial properties against a range of bacterial strains implicated in ocular infections. The research findings demonstrate that stem extracts derived from Buddleja polystachya demonstrated high to moderate cytotoxic effects on cancer cell lines MCF7, HT29, and HepG2. Notably, these effects were characterized by varying IC values, which suggest distinct levels of sensitivity. In contrast, leaf extracts exhibited reduced cytotoxicity when tested against all these cell lines, although they did so with a significantly higher cytotoxicity aganist HepG2 cells. The results of this investigation highlight the potential therapeutic utilization of Buddleja polystachya extracts in the management of ocular infections and cancer. These results support the need for additional research to elucidate the underlying mechanisms of action of these extracts and explore their potential as drugs.
这项对多穗醉鱼草的研究突出了其植物化学成分、抗菌活性和细胞毒性影响。该研究通过气相色谱 - 质谱分析确定参与生物活性的重要化合物，强调了这种植物治疗眼部疾病的潜力。本研究探讨了多穗醉鱼草（茎和叶）提取物的抗菌和细胞毒性潜力，重点关注其在治疗细菌性眼部感染中的应用以及对MCF7、HT29和HepG2癌细胞的疗效。通过全面的气相色谱 - 质谱分析，在多穗醉鱼草茎和叶提取物中鉴定出了多种植物化学物质，包括碳水化合物、酚类衍生物、脂肪酸和甾体成分。然后对提取物的生物活性进行评估，结果显示其对一系列与眼部感染有关的细菌菌株具有显著的抗菌特性。研究结果表明，多穗醉鱼草的茎提取物对癌细胞系MCF7、HT29和HepG2表现出高至中等的细胞毒性作用。值得注意的是，这些作用的特征是具有不同的IC值，这表明敏感性水平不同。相比之下，叶提取物在针对所有这些细胞系进行测试时表现出较低的细胞毒性，尽管它们对HepG2细胞的细胞毒性明显更高。这项调查的结果突出了多穗醉鱼草提取物在眼部感染和癌症治疗中的潜在治疗用途。这些结果支持需要进行更多研究以阐明这些提取物的潜在作用机制，并探索它们作为药物的潜力。
