Division of Molecular bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Berngrid.5734.5, Bern, Switzerland.
Graduate School of Cellular and Biomedical Sciences, University of Berngrid.5734.5, Bern, Switzerland.
Microbiol Spectr. 2022 Oct 26;10(5):e0312122. doi: 10.1128/spectrum.03121-22. Epub 2022 Sep 26.
A novel multidrug resistance conjugative 177,859-bp IncC plasmid pJEF1-OXA-181 coharboring the carbapenemase-coding and the aminoglycoside resistance 16S rRNA methyltransferase-coding genes was detected in two unrelated Escherichia coli gut isolates of ST196 and ST648, as well as two ST35 Klebsiella pneumoniae gut and sputum isolates of a cystic fibrosis patient. The gene was located within the antimicrobial resistance island ARI-A and the gene, which was preceded by IS and IS was inserted within the transfer genes region without affecting conjugation ability. Comparative plasmid analysis with other related IncC plasmids showed the presence of , as well as its integration site, are thus far unique for these types of plasmids. This study illustrates the potential of a promiscuous multidrug resistance plasmid to acquire antibiotic resistance genes and to disseminate in the gut of the same host. Colocalization of carbapenemases and aminoglycoside resistance 16S rRNA methylases on a multidrug resistance conjugative plasmid poses a serious threat to public health. Here, we describe the novel IncC plasmid pJEF1-OXA-181 cocarrying and as well as several other antimicrobial resistance genes (ARGs) in different Enterobacterales isolates of the sputum and gut microbiota of a cystic fibrosis patient. IncC plasmids are conjugative, promiscuous elements which can incorporate accessory antimicrobial resistance islands making them key players in ARGs spread. This plasmid was thus far unique among IncC plasmids to contain a which was integrated in the transfer gene region without affecting its conjugation ability. This study highlights that new plasmids may be introduced into a hospital through different species hosted in one single patient. It further emphasizes the need of continuous surveillance of multidrug-resistant bacteria in patients at risk to avoid spread of such plasmids in the health care system.
一种新型的 177859 碱基对的可移动多药耐药性 IncC 质粒 pJEF1-OXA-181 同时携带碳青霉烯酶编码基因和氨基糖苷类抗性 16S rRNA 甲基转移酶编码基因，在两个无关的大肠杆菌肠道分离株 ST196 和 ST648 中以及两个 ST35 型肺炎克雷伯菌肠道和肺部分离株中被检测到，该分离株来自于一名囊性纤维化患者。基因位于抗生素耐药性岛 ARI-A 内，而基因则被 IS 和 IS 插入在转移基因区域内，而不会影响其接合能力。与其他相关 IncC 质粒的比较质粒分析表明，基因及其整合位点在这些类型的质粒中是独特的。本研究说明了一种混杂的多药耐药性质粒具有获取抗生素耐药基因并在同一宿主的肠道中传播的潜力。碳青霉烯酶和氨基糖苷类抗性 16S rRNA 甲基转移酶在一个多药耐药性可移动质粒上的共存对公共健康构成了严重威胁。在这里，我们描述了一种新型的 IncC 质粒 pJEF1-OXA-181，它在一名囊性纤维化患者的痰液和肠道微生物群的不同肠杆菌科分离株中共同携带基因和基因，以及其他几种抗生素耐药基因（ARGs）。IncC 质粒是可移动的、混杂的元件，可以整合辅助抗生素耐药性岛，使其成为 ARGs 传播的关键因素。到目前为止，这种质粒在 IncC 质粒中是独特的，它包含一个整合在转移基因区域内而不影响其接合能力的基因。本研究强调，新的质粒可能通过宿主在一个单一患者中引入到医院中。它进一步强调了需要对处于风险中的患者中的多药耐药菌进行持续监测，以避免此类质粒在医疗保健系统中的传播。
