Fu Hongyu, Zhu Zhichen, Wang Xiao, Lv Jingnan, Zhu Jie, Chen Liang, Yu Hua, Du Hong
Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Department of Blood Transfusion, The Children's Hospital of Soochow University, Suzhou, Jiangsu, China.
Front Microbiol. 2024 Aug 14;15:1404996. doi: 10.3389/fmicb.2024.1404996. eCollection 2024.
was defined as a novel species in the genus. in 2019, however, antimicrobial resistance, such as carbapenem resistance, has rarely been described in . This study described the molecular features of four carbapenem-resistant strains collected from a tertiary health care hospital in Southwest China.
Whole genome sequencing (WGS) was used to determine the genome sequence of four strains. The precise species of strains were identified by average nucleotide identity (ANI) and DNA-DNA hybridization (isDDH). The clonal relatedness of four strains and additional 15 ones from NCBI were examined through phylogenetic analysis. The molecular features of and genetic structure of carbapenemase- encoding plasmids were characterized through genomic annotation and analysis.
The results revealed the emergence of -carrying strains in China. Multilocus sequence typing (MLST) analysis showed that all 19 belonged to the same sequence type of ST414. Core SNP analysis suggested the potential intrahospital clonal transmission of ST414 . The carbapenemase-encoding gene was harbored by an IncC-type plasmid, which was experimentally confirmed to be able to conjugate.
This study reports the first emergence and potential clonal transmission of -carrying . Further surveillance should be advocated to monitor the dissemination of carbapenem-resistant and -harboring IncC-type plasmids in China.
在2019年被定义为该属中的一个新物种。然而，很少有关于其耐药性的描述，比如碳青霉烯类耐药性。本研究描述了从中国西南部一家三级医疗机构收集的4株耐碳青霉烯类菌株的分子特征。
使用全基因组测序（WGS）来确定4株菌株的基因组序列。通过平均核苷酸同源性（ANI）和数字化DNA-DNA杂交（isDDH）鉴定菌株的确切种类。通过系统发育分析检查了4株菌株与来自NCBI的另外15株菌株的克隆相关性。通过基因组注释和分析对携带碳青霉烯酶的质粒的分子特征和遗传结构进行了表征。
结果揭示了中国携带的菌株的出现。多位点序列分型（MLST）分析表明，所有19株均属于ST414的同一序列类型。核心SNP分析表明ST414菌株可能存在医院内克隆传播。携带碳青霉烯酶的基因由IncC型质粒携带，经实验证实该质粒能够接合。
本研究报告了携带的首次出现和潜在的克隆传播。应提倡进一步监测，以监测耐碳青霉烯类和携带的IncC型质粒在中国的传播情况。
