Wu Changrong, Sun Wentao, Huang Yuan, Dai Sheng, Peng Chao, Zheng Yulong, Chen Chao, Hao Jun
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, China.
Front Microbiol. 2022 Sep 8;13:904193. doi: 10.3389/fmicb.2022.904193. eCollection 2022.
The purpose of this study was to investigate the effects of inoculation with two lactic acid bacteria (LAB) strains ( and ) and the addition of four corn flour proportions (0, 3, 6, and 9%) in different treatments, on the composition and function of the bacterial community in whole-plant paper mulberry silage. The different treatments promoted , , and growth, but the microbial species responsible for fermentation differed among the treatments. High species diversity and various Gram-negative bacteria, such as sp., , , , and , were found in the uninoculated LAB treatments. A beneficial bacterium, , was discovered for the first time in whole-plant paper mulberry silage. LAB inoculation simplifies the microbial community structure, and beneficial as a key species aggregates in the inoculated treatment group. However, inoculation alone may have limited bacteriostatic activity against in whole-plant paper mulberry silage. Compared with silage lacking corn flour, amino sugar and nucleotide sugar metabolism, galactose metabolism, the phosphotransferase system and the pentose phosphate pathway metabolic activity were increased in corn flour-containing silage. Whole-plant paper mulberry can be used as a high-quality silage to provide high-quality feed resources for sustainable ruminant livestock production. Moreover, additive use is necessary for preparing paper mulberry silage.
本研究的目的是调查接种两种乳酸菌（LAB）菌株（ 和 ）以及在不同处理中添加四种玉米粉比例（0%、3%、6%和9%）对全株构树青贮饲料中细菌群落组成和功能的影响。不同处理促进了 、 和 的生长，但负责发酵的微生物种类在各处理之间有所不同。在未接种LAB的处理中发现了高物种多样性以及各种革兰氏阴性菌，如 菌属、 、 、 和 。在全株构树青贮饲料中首次发现了一种有益细菌 。接种LAB简化了微生物群落结构，并且有益菌 作为关键物种聚集在接种处理组中。然而，单独接种 在全株构树青贮饲料中可能具有有限的抑菌活性。与不含玉米粉的青贮饲料相比，含玉米粉的青贮饲料中氨基糖和核苷酸糖代谢、半乳糖代谢、磷酸转移酶系统和戊糖磷酸途径的代谢活性有所增加。全株构树可用作优质青贮饲料，为可持续反刍家畜生产提供优质饲料资源。此外，制备构树青贮饲料需要使用添加剂。
