FOXP3+ 调节性 T 细胞对局部晚期口咽鳞状细胞癌患者的预后价值。
Prognostic value of FOXP3+ regulatory T cells for patients with locally advanced oropharyngeal squamous cell carcinoma.
机构信息
Division of Hematology and Oncology, Department of Internal Medicine, Hanyang University Guri Hospital, Guri, Republic of Korea.
Research Institute for Future Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
出版信息
PLoS One. 2022 Oct 6;17(10):e0274830. doi: 10.1371/journal.pone.0274830. eCollection 2022.
BACKGROUND
Oropharyngeal squamous cell carcinoma (OPSCC) is the most common neoplasm originating at the base of the tongue or in the tonsils or soft palate. In this study, we investigated the prognostic value of FOXP3+ regulatory T cells in OPSCC.
METHODS
Tumor tissues of patients with locally advanced OPSCC were analyzed using quantitative multiplex immunohistochemistry. Staining of CD8+ T cells, conventional CD4+FOXP3- T cells (Tconv cells), CD4+FOXP3+ regulatory T cells (Treg cells), CD20+ B cells, and CD68+ macrophages was performed, and cell density was evaluated in both the tumor and its stroma.
RESULTS
Among the 71 patients included in this study, males constituted 93.0% of the cohort, and the median age was 59 years (range: 42-80 years). A total of 56 patients (78.9%) had a smoking history, and 53 (74.6%) patients were positive for human papillomavirus (HPV). The most frequent site of OPSCC was the tonsils (70.4%), followed by the base of the tongue (25.4%). The proportion of Treg cells was lower in the tumors of patients with HPV than in those of patients without HPV. Patients with OPSCC whose tumor Treg cell levels were above the median had longer relapse-free survival (RFS) periods than those with tumor Treg cell levels below the median (HR, 0.12; 95% CI, 0.03-0.46; p = 0.02). Our multivariate analysis identified high Treg levels (HR, 0.13; 95% CI, 0.02-1.00; p = 0.05) as an RFS factor that predicted a good prognosis.
CONCLUSIONS
Our results demonstrated that high Treg cell density in locally advanced OPSCC tumors was correlated with longer RFS.
背景
口咽鳞状细胞癌（OPSCC）是最常见的起源于舌根或扁桃体或软腭的肿瘤。在本研究中，我们研究了 FOXP3+调节性 T 细胞在 OPSCC 中的预后价值。
方法
使用定量多重免疫组织化学分析局部晚期 OPSCC 患者的肿瘤组织。对 CD8+T 细胞、常规 CD4+FOXP3-T 细胞（Tconv 细胞）、CD4+FOXP3+调节性 T 细胞（Treg 细胞）、CD20+B 细胞和 CD68+巨噬细胞进行染色，并评估肿瘤及其基质中的细胞密度。
结果
在本研究纳入的 71 例患者中，男性占队列的 93.0%，中位年龄为 59 岁（范围：42-80 岁）。共有 56 例（78.9%）有吸烟史，53 例（74.6%）患者 HPV 阳性。OPSCC 最常见的部位是扁桃体（70.4%），其次是舌根（25.4%）。HPV 阳性患者肿瘤中 Treg 细胞的比例低于 HPV 阴性患者。肿瘤 Treg 细胞水平高于中位数的 OPSCC 患者的无复发生存期（RFS）长于肿瘤 Treg 细胞水平低于中位数的患者（HR，0.12；95%CI，0.03-0.46；p=0.02）。我们的多变量分析确定高 Treg 水平（HR，0.13；95%CI，0.02-1.00；p=0.05）是预测预后良好的 RFS 因素。
结论
我们的结果表明，局部晚期 OPSCC 肿瘤中 Treg 细胞密度高与 RFS 延长相关。
Zotero 插件