Genos Glycoscience Research Laboratory, Zagreb, Croatia.
Centre for Healthy Weigh, The Novo Nordisk Foundation, Hellerup, Denmark.
Front Immunol. 2022 Sep 21;13:995186. doi: 10.3389/fimmu.2022.995186. eCollection 2022.
Obesity-induced inflammation activates the adaptive immune system by altering immunoglobulin G (IgG) glycosylation in a way to produce more proinflammatory antibodies. The IgG glycome has already been well studied, and its alterations are correlated with a high body mass index (BMI) and central adiposity. Still, the IgG N-glycome susceptibility to different dietary regimes for weight control after the initial weight loss has not been studied. To explore changes in IgG glycosylation induced by weight loss and subsequent weight-maintenance diets, we analyzed 1,850 IgG glycomes from subjects in a dietary intervention Diogenes study. In this study, participants followed a low-calorie diet (LCD) providing 800 kcal/d for 8 weeks, followed by one of five weight-maintenance diets over a 6-month period. The most significant alteration of the IgG N-glycome was present 8 weeks after the subjects underwent an LCD, a statistically significant decrease of agalactosylated and the increase of sialylated N glycans. In the follow-up period, the increase in glycans with bisecting GlcNAc and the decrease in sialylated glycans were observed. Those changes were present regardless of the diet type, and we did not observe significant changes between different diets. However, it should be noted that in all five diet groups, there were individuals who prominently altered their IgG glycome composition in either proinflammatory or anti-inflammatory directions.
肥胖引起的炎症通过改变免疫球蛋白 G（IgG）糖基化的方式激活适应性免疫系统，从而产生更多促炎抗体。IgG 聚糖已得到充分研究，其改变与高体重指数（BMI）和中心性肥胖相关。然而，对于初始体重减轻后控制体重的不同饮食方案对 IgG N-聚糖的影响尚未进行研究。为了探讨体重减轻和随后的维持体重饮食引起的 IgG 糖基化变化，我们分析了 Diogenes 饮食干预研究中 1850 个 IgG 聚糖。在这项研究中，参与者遵循低热量饮食（LCD），每天提供 800 卡路里，持续 8 周，然后在 6 个月内遵循 5 种维持体重的饮食之一。在参与者接受 LCD 后 8 周，观察到 IgG N-聚糖的最显著改变，即半乳糖基化的 IgG N-聚糖显著减少，唾液酸化的 IgG N-聚糖增加。在随后的随访期间，观察到具有双连接 GlcNAc 的 IgG N-聚糖增加和唾液酸化的 IgG N-聚糖减少。这些变化与饮食类型无关，并且我们在不同的饮食之间没有观察到显著的变化。然而，应当注意的是，在所有 5 种饮食组中，都有个体的 IgG 聚糖组成朝着促炎或抗炎方向明显改变。
