Department of Chemistry, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey.
Department of Chemistry, Engineering Faculty, Istanbul University-Cerrahpasa, Avcilar, 34320, Istanbul, Turkey.
Mol Biotechnol. 2023 May;65(5):786-793. doi: 10.1007/s12033-022-00581-2. Epub 2022 Oct 10.
In recent years, within tissue engineering, cell growth on patterned surfaces have gained significant attention. Growing cells in patterns is important to manufacture polymeric tissues that can be used within the medical field. For this reason, the main focus of this study was to prepare patterned scaffolds using Titanium (Ti) and polyvinyl chloride (PVC) covered on microscope lamellas and examine their liability for cell growth. A polydimethylsiloxane stamp was initially prepared which was then used to transfer a predefined pattern onto PVC- and Ti-covered surfaces. Cell growth experiments were performed on the prepared materials by seeding L929 mouse fibroblasts. The growth of cells seeded on the surface of the scaffolds were spectroscopically followed using Neutral Red uptake assay. The results showed cell proliferation on both patterned surfaces, however, it was higher on Ti-covered samples. In addition, three different alkanethiols were tested for cell adhesion on patterned surfaces. A higher number of cell proliferation was observed with undecanethiol, which has a shorter alkane group among them. The morphological properties of the samples before and after cell-seeding were analyzed via scanning electron microscope and optical microscopy. Significant amount of cell proliferation was observed on all of the prepared samples.
近年来，在组织工程领域，细胞在图案化表面上的生长引起了广泛关注。在图案中培养细胞对于制造可用于医学领域的聚合物组织非常重要。出于这个原因，本研究的主要重点是使用涂覆在显微镜薄片上的钛（Ti）和聚氯乙烯（PVC）来制备图案化支架，并研究其对细胞生长的适用性。首先制备了聚二甲基硅氧烷（PDMS）印章，然后将其用于将预定义的图案转移到涂覆有 PVC 和 Ti 的表面上。通过在种子 L929 小鼠成纤维细胞上接种来在制备的材料上进行细胞生长实验。使用中性红摄取测定法光谱学跟踪接种在支架表面上的细胞的生长情况。结果表明，细胞在两种图案化表面上均增殖，但在涂覆 Ti 的样品上增殖更高。此外，还测试了三种不同的烷硫醇在图案化表面上的细胞粘附性。在它们中，具有较短烷烃基团的十一烷硫醇观察到更高数量的细胞增殖。通过扫描电子显微镜和光学显微镜分析了细胞接种前后样品的形态特性。在所有制备的样品上均观察到大量的细胞增殖。
