提高植物中碱基编辑器的编辑效率。
Improving editing efficiency of prime editor in plants.
作者信息
Ahmad Niaz, Awan Muhammad Jawad Akbar, Mansoor Shahid
机构信息
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College (NIBGE-C), Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College (NIBGE-C), Pakistan Institute of Engineering and Applied Sciences, Jhang Road, Faisalabad, Pakistan.
出版信息
Trends Plant Sci. 2023 Jan;28(1):1-3. doi: 10.1016/j.tplants.2022.09.001. Epub 2022 Oct 7.
Prime editing creates targeted insertions or deletions in the genome without double-stranded breaks (DSBs). However, prime-editing efficiency in plants is low, thereby limiting its utilization. A recent study by Zong et al. used a simple strategy that led to substantially improvements in the editing efficiency in both protoplasts and transgenic plants.
引导编辑可在基因组中实现靶向插入或缺失，而无需产生双链断裂（DSB）。然而，植物中的引导编辑效率较低，从而限制了其应用。宗等人最近的一项研究采用了一种简单的策略，使得原生质体和转基因植物中的编辑效率都有了显著提高。
Zotero 插件