Dos Santos Gabriel Grube, Malherbi Milena Schroeder, de Souza Natália Silva, César Gabriel Batista, Tominaga Tania Toyomi, Miyahara Ricardo Yoshimitsu, de Mendonça Patrícia de Souza Bonfim, Faria Daniela Renata, Rosso Jaciele Márcia, Freitas Valdirlei Fernandes, Weinand Wilson Ricardo, Dias Gustavo Sanguino, Santos Ivair Aparecido, Cotica Luiz Fernando, Bonadio Taiana Gabriela Moretti
Graduate Program in Applied Chemistry, Midwestern Paraná State University, Guarapuava 85040167, PR, Brazil.
Department of Physics, Midwestern Paraná State University, Guarapuava 85040167, PR, Brazil.
Polymers (Basel). 2022 Oct 6;14(19):4190. doi: 10.3390/polym14194190.
Biomaterials that effectively act in biological systems, as in treatment and healing of damaged or lost tissues, must be able to mimic the properties of the body's natural tissues in its various aspects (chemical, physical, mechanical and surface). These characteristics influence cell adhesion and proliferation and are crucial for the success of the treatment for which a biomaterial will be required. In this context, the electrospinning process has gained prominence in obtaining fibers of micro- and nanometric sizes from polymeric solutions aiming to produce scaffolds for tissue engineering. In this manuscript, poly(vinylidene fluoride) (PVDF) was used as a polymeric matrix for the manufacture of piezoelectric scaffolds, exploring the formation of the β-PVDF piezoelectric phase. Micro- and nanometric hydroxyapatite (HA) particles were incorporated as a dispersed phase in this matrix, aiming to produce multifunctional composite membranes also with bioactive properties. The results show that it is possible to produce membranes containing micro- and nanofibers of the composite by the electrospinning process. The HA particles show good dispersion in the polymer matrix and predominance of β-PVDF phase. Also, the composite showed apatite growth on its surface after 21 days of immersion in simulated body fluid (SBF). Tests performed on human fibroblasts culture revealed that the electrospun membranes have low cytotoxicity attesting that the composite shows great potential to be used in biomedical applications as bone substitutions and wound healing.
能在生物系统中有效发挥作用的生物材料，如用于受损或缺失组织的治疗与修复，必须能够在各个方面（化学、物理、机械和表面）模拟人体天然组织的特性。这些特性会影响细胞的黏附和增殖，对于需要使用生物材料的治疗的成功至关重要。在这种背景下，静电纺丝工艺在从聚合物溶液中获得微米级和纳米级尺寸的纤维以生产用于组织工程的支架方面受到了关注。在本手稿中，聚偏氟乙烯（PVDF）被用作制造压电支架的聚合物基体，探索β-PVDF压电相的形成。微米级和纳米级羟基磷灰石（HA）颗粒作为分散相被掺入该基体中，旨在生产同样具有生物活性的多功能复合膜。结果表明，通过静电纺丝工艺可以生产出含有该复合材料的微米级和纳米级纤维的膜。HA颗粒在聚合物基体中显示出良好的分散性以及β-PVDF相的优势。此外，该复合材料在模拟体液（SBF）中浸泡21天后，其表面出现了磷灰石生长。对人成纤维细胞培养进行的测试表明，静电纺丝膜具有低细胞毒性，证明该复合材料在作为骨替代物和伤口愈合的生物医学应用中具有巨大潜力。
