Yan Xinlei, Sun Yufei, Zhang Guangzhi, Han Wenying, Gao Jialu, Yu Xiuli, Jin Xindong
Food Science and Engineering College of Inner Mongolia Agricultural University, Hohhot, China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Nutr. 2022 Sep 28;9:1014344. doi: 10.3389/fnut.2022.1014344. eCollection 2022.
is an important food-borne zoonotic parasite, and approximately one-third of people worldwide are positive for antibodies. To date, there are no specific drugs or vaccines against . Therefore, developing a new safe and effective method has become a new trend in treating toxoplasmosis. Koumiss is rich in probiotics and many components that can alleviate the clinical symptoms of many diseases the functional characteristics of koumiss and its regulation of intestinal flora. To investigate the antagonistic effect of koumiss on infection, the model of acute and chronic infection was established in this study. The survival rate, SHIRPA score, serum cytokine levels, brain cyst counts, β-amyloid deposition and intestinal flora changes were measured after koumiss feeding. The results showed that the clinical symptoms of mice were improved at 6 dpi and that the SHIRPA score decreased after koumiss feeding ( < 0.05). At the same time, the levels of IL-4, IFN-γ and TNF-α decreased ( < 0.001, < 0.001, < 0.01). There was no significant difference of survival rate between koumiss treatment and the other groups. Surprisingly, the results of chronic infection models showed that koumiss could significantly reduce the number of brain cysts in mice ( < 0.05), improve β-amyloid deposition in the hippocampus ( < 0.01) and decrease the levels of IFN-γ and TNF-α ( < 0.01, < 0.05). Moreover, koumiss could influence the gut microbiota function in resisting infection. In conclusion, koumiss had a significant effect on chronic infection in mice and could improve the relevant indicators of acute infection in mice. The research provides new evidence for the development of safe and effective anti- methods, as well as a theoretical basis and data support for the use of probiotics against infection and broadened thoughts for the development and utilization of koumiss.
是一种重要的食源性人畜共患寄生虫，全球约三分之一的人抗体呈阳性。迄今为止，尚无针对的特异性药物或疫苗。因此，开发一种新的安全有效的方法已成为治疗弓形虫病的新趋势。马奶酒富含益生菌和许多可缓解多种疾病临床症状的成分，研究马奶酒的功能特性及其对肠道菌群的调节作用。为研究马奶酒对感染的拮抗作用，本研究建立了急性和慢性感染模型。在喂食马奶酒后测量存活率、SHIRPA评分、血清细胞因子水平、脑囊肿计数、β-淀粉样蛋白沉积和肠道菌群变化。结果显示，感染后6天小鼠的临床症状得到改善，喂食马奶酒后SHIRPA评分降低（<0.05）。同时，IL-4、IFN-γ和TNF-α水平降低（<0.001，<0.001，<0.01）。马奶酒治疗组与其他组的存活率无显著差异。令人惊讶的是，慢性感染模型的结果表明，马奶酒可显著减少小鼠脑囊肿数量（<0.05），改善海马体中的β-淀粉样蛋白沉积（<0.01），并降低IFN-γ和TNF-α水平（<0.01，<0.05）。此外，马奶酒可影响肠道微生物群抵抗感染的功能。总之，马奶酒对小鼠慢性感染有显著影响，可改善小鼠急性感染的相关指标。该研究为开发安全有效的抗方法提供了新证据，也为益生菌用于抗感染提供了理论依据和数据支持，并拓宽了马奶酒开发利用的思路。
