Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt.
Botany and Microbiology Department, Faculty of Science, New Valley University, El Kharja, Egypt.
BMC Plant Biol. 2022 Oct 17;22(1):490. doi: 10.1186/s12870-022-03790-8.
Evaluation of native soil microbes is a realistic way to develop bio-agents for ecological restoration. Soil alkalinity, which has a high pH, is one of the most common concerns in dry and semi-arid climates. Alkaline soils face problems due to poor physical properties, which affect plant growth and crop production. A pot experiment was carried out to investigate the impact of native mycorrhizal fungi (AMF) on the wheat plant (Triticum aestivum L.) under two levels of alkalinity stress -T1 (37 mM NaHCO), T2 (74 mM NaHCO) - at two developmental stages (the vegetative and productive stages).
Alkalinity stress significantly inhibited the germination percentage, plant biomass, photosynthetic pigments, and some nutrients (K, N, and P). Mycorrhizal inoculation improved growth parameters and productivity of wheat-stressed plants. However, lipid peroxidation was significantly lowered in mycorrhizal-inoculated plants compared to non-inoculated plants. Catalase and peroxidase were inhibited in wheat leaves and roots by alkalinity, while mycorrhiza promoted the activity of these enzymes.
The results of this study demonstrated that alkalinity stress had highly negative effects on some growth parameters of the wheat plant, while AMF inoculation attenuated these detrimental effects of alkalinity stress at two stages by reducing the pH and Na concentration and increasing the availability of P and the productivity of wheat in particular crop yield parameters.
评估本地土壤微生物是开发生态修复生物制剂的现实途径。土壤碱性是干旱和半干旱气候下最常见的问题之一，其 pH 值较高。碱性土壤由于物理性质较差而面临问题，这会影响植物生长和作物产量。进行了一项盆栽实验，以研究在两种碱胁迫水平下（T1（37 mM NaHCO3）和 T2（74 mM NaHCO3））和两个发育阶段（营养和生殖阶段）下，本地菌根真菌（AMF）对小麦（Triticum aestivum L.）植物的影响。
碱胁迫显著抑制了发芽率、植物生物量、光合色素和一些养分（K、N 和 P）。菌根接种提高了受胁迫植物的生长参数和生产力。然而，与未接种植物相比，菌根接种植物的脂质过氧化显著降低。碱胁迫抑制了小麦叶片和根中的过氧化氢酶和过氧化物酶活性，而菌根则促进了这些酶的活性。
本研究结果表明，碱胁迫对小麦植物的一些生长参数有高度负面影响，而 AMF 接种通过降低 pH 值和 Na 浓度、增加 P 的有效性以及提高小麦特别是作物产量参数的生产力，减轻了碱胁迫的这些不利影响。
