Chan L F, Chhuy L H, Crowley R J
J Anal Toxicol. 1987 Jul-Aug;11(4):171-4. doi: 10.1093/jat/11.4.171.
Postmortem findings are presented for fourteen cases of Verapamil induced or related deaths. Liver, blood, and stomach concentrations of Verapamil alone or in combination with alcohol or other drugs are given with the age and sex of each victim and with the coroner's findings. The significance of these levels is discussed. Tissue homogenates were digested either by strong acid or enzymically by subtilisin and extracted with dichloromethane or 1-chlorobutane, respectively. Blood was buffered to pH 9 and extracted directly with 1-chlorobutane. Recoveries are reported. Verapamil was quantitated by gas chromatography on a 3% OV-17 column with a nitrogen-phosphorus detector.
本文呈现了14例维拉帕米诱导或相关死亡病例的尸检结果。给出了每位受害者的年龄、性别以及验尸官的调查结果，同时还列出了单独使用维拉帕米或与酒精或其他药物联合使用时，肝脏、血液和胃中的维拉帕米浓度。讨论了这些浓度的意义。组织匀浆分别用强酸消化或用枯草杆菌蛋白酶酶解，然后分别用二氯甲烷或1-氯丁烷萃取。血液用缓冲液调节至pH 9，然后直接用1-氯丁烷萃取。报告了回收率。维拉帕米通过气相色谱法在3% OV-17柱上用氮磷检测器进行定量分析。
