Department of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou, China.
School of Stomatology, Northwest Minzu University, Lanzhou, China.
Front Cell Infect Microbiol. 2022 Oct 4;12:943808. doi: 10.3389/fcimb.2022.943808. eCollection 2022.
Emerging evidence revealed that the blood microbiota plays a role in several non-communicable diseases, including cardiovascular disease. However, the role of circulating microbes in atherosclerosis remains understudied. To test this hypothesis, we performed this study to investigate the microbial profile in the blood of Chines atherosclerosis volunteers. A total of seventy Acute Coronary Syndrome patients, seventy Chronic Coronary Syndrome patients, and seventy healthy individuals were examined using high-throughput Illumina Novaseq targeting the V3-V4 regions of the 16S rRNA gene. The relationship between atherosclerosis and blood microbiome, clinical variables, and their functional pathways were also investigated. Our study observed significantly higher alpha diversity indices (Chao1, = 0.001, and Shannon, = 0.004) in the acute coronary syndrome group compared with chronic coronary syndrome and healthy group, although a significantly lower alpha diversity was observed in the chronic coronary syndrome compared to acute coronary syndrome and healthy group. Beta diversity based on principal coordinate analysis demonstrated a major separation among the three groups. In addition, using linear discriminant analysis, a significant distinct taxon such as Actinobacteria _ phylum, and genus in the healthy group; Firmicutes_ phylum, and _ genus in the chronic coronary syndrome group, and Proteobacteria and Acidobacteriota _ phyla in acute coronary syndrome group were observed among three groups. Clusters of Orthologous Genes grouped and Kyoto Encyclopedia of Genes and Genomes pathways suggested a significant variation among all groups (). The blood microbiota analysis provides potential biomarkers for the detection of coronary syndromes in this population.
新出现的证据表明，血液微生物群在包括心血管疾病在内的几种非传染性疾病中发挥作用。然而，循环微生物在动脉粥样硬化中的作用仍未得到充分研究。为了验证这一假说，我们进行了这项研究，以调查中国动脉粥样硬化志愿者血液中的微生物特征。我们使用高通量 Illumina Novaseq 靶向 16S rRNA 基因的 V3-V4 区域，共检测了 70 名急性冠状动脉综合征患者、70 名慢性冠状动脉综合征患者和 70 名健康个体。还研究了动脉粥样硬化与血液微生物组、临床变量及其功能途径之间的关系。我们的研究观察到，与慢性冠状动脉综合征和健康组相比，急性冠状动脉综合征组的 alpha 多样性指数（Chao1，= 0.001，和 Shannon，= 0.004）显著更高，尽管慢性冠状动脉综合征组的 alpha 多样性明显低于急性冠状动脉综合征组和健康组。基于主坐标分析的 beta 多样性表明三组之间存在主要分离。此外，使用线性判别分析，在健康组中观察到显著不同的分类群，如放线菌门和属；在慢性冠状动脉综合征组中观察到厚壁菌门和属；在急性冠状动脉综合征组中观察到变形菌门和酸杆菌门。同源基因聚类和京都基因与基因组百科全书途径表明所有组之间存在显著差异（）。血液微生物群分析为该人群中冠状动脉综合征的检测提供了潜在的生物标志物。
