Shioya M, Wakabayashi K, Sato S, Nagao M, Sugimura T
Mutat Res. 1987 Jul-Aug;191(3-4):133-8. doi: 10.1016/0165-7992(87)90143-6.
When a mixture of creatinine, phenylalanine and glucose in diethylene glycol-water solution was heated for 2 h at 128 degrees C, a mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), was found to be produced, identified by high-performance liquid chromatography, and UV-visible and mass spectrometries. The yield of PhIP was 3.6 nmoles/mmole original creatinine. PhIP was originally isolated from cooked beef, and this study shows creatinine, phenylalanine and glucose to be the probable precursors of PhIP in beef.
当将肌酸酐、苯丙氨酸和葡萄糖在二甘醇 - 水溶液中的混合物于128℃加热2小时时，发现会产生一种诱变剂，即2 - 氨基 - 1 - 甲基 - 6 - 苯基咪唑并[4,5 - b]吡啶（PhIP），通过高效液相色谱、紫外 - 可见光谱和质谱法进行了鉴定。PhIP的产量为3.6纳摩尔/毫摩尔原始肌酸酐。PhIP最初是从熟牛肉中分离出来的，这项研究表明肌酸酐、苯丙氨酸和葡萄糖可能是牛肉中PhIP的前体。
